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Create a diagram of the steps in the Most Probable Number test. You may insert a computer-made diagram, or you may draw a diagram by hand

Create a diagram of the steps in the Most Probable Number test. You may insert a computer-made diagram, or you may draw a diagram by hand

Principles Of Pharmacology Med Assist
Principles Of Pharmacology Med Assist
6th Edition
ISBN:9781337512442
Author:RICE
Publisher:RICE
Chapter8: Forms Of Drugs And How They Act
Section: Chapter Questions
Problem 30RQ
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Create a diagram of the steps in the Most Probable Number test. You may insert a computer-made diagram, or you may draw a diagram by hand
12:47 e O A. Bacteriological analysis of water: Most Probable Number (MPN) technique 1. Students should work in groups of 3 (side of a bench) and use ONE water sample provided by them. 2. Using the diagram below as a guide, aseptically pipette 10 ml of the sample into each of three* 10 ml double strength lactose Durham tubes; pipette 1 ml of the sample into each of three 9 ml single strength lactose Durham tubes and finally, pipette 0.1 ml of the sample into each of three 9 ml single strength Durham tubes. 3. Gently shake all tubes and incubate at 37°C for 48 hrs. double strength lactose-pipette 10 ml sample single strength lactose--pipette 1.0 ml sample single strength lactose-pipette 0.1 water sample I sample *Normally 5 Durham tubes are inoculated but this exercise is modified to three in the interest of economy. 4. Record the number after 48 hrs of continuous incubation. Consult Table 1 and determine the MPN for your tubes each of the three dilutions of the sample that show gas sample. 5. For each presumptive positive tube, streak an EMB plate for isolated colonies. 6. Record the Completed test MPN results. (Note that we are skipping the confirmed test). 7. Observe the demonstrations of the completed tests of the MPN. Be able to state how and why each test is performed. Questions: 1. Define coliform, fecal coliform and indicator organism. 2. Why are coliforms and in particular, E. coli, "ideal indicators" of water contamination? II
Transcribed Image Text:12:47 e O A. Bacteriological analysis of water: Most Probable Number (MPN) technique 1. Students should work in groups of 3 (side of a bench) and use ONE water sample provided by them. 2. Using the diagram below as a guide, aseptically pipette 10 ml of the sample into each of three* 10 ml double strength lactose Durham tubes; pipette 1 ml of the sample into each of three 9 ml single strength lactose Durham tubes and finally, pipette 0.1 ml of the sample into each of three 9 ml single strength Durham tubes. 3. Gently shake all tubes and incubate at 37°C for 48 hrs. double strength lactose-pipette 10 ml sample single strength lactose--pipette 1.0 ml sample single strength lactose-pipette 0.1 water sample I sample *Normally 5 Durham tubes are inoculated but this exercise is modified to three in the interest of economy. 4. Record the number after 48 hrs of continuous incubation. Consult Table 1 and determine the MPN for your tubes each of the three dilutions of the sample that show gas sample. 5. For each presumptive positive tube, streak an EMB plate for isolated colonies. 6. Record the Completed test MPN results. (Note that we are skipping the confirmed test). 7. Observe the demonstrations of the completed tests of the MPN. Be able to state how and why each test is performed. Questions: 1. Define coliform, fecal coliform and indicator organism. 2. Why are coliforms and in particular, E. coli, "ideal indicators" of water contamination? II
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