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- 5. a) Thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) can be used asexamples to explain difference between adsorption and partition. For each of these chromatographic techniques,identify:+ the stationary and mobile phase+ how the mobile phase movesb) How is the Rf value for a spot on a TLC plate calculated? What can the Rf value be used for? In order toachieve the best separation of several components of different polarity in a mixture spotted on a TLC plate,what guideline should one follow regarding the choice of a developing solvent? (i.e. polar, non-polar, etc.)1) give detail note ( giving points ) on three generation of photosensitizer (PSS) with example and also highlight the role of third generation photosensitizer . [ please refer research paper to give answer at end end of the answer please write the DOI number ]Briefly explain why the 14C/12C ratio will decrease after the death of plants. A three- to four-sentence explanation is needed.
- In UV/Visible spectrophotometer analysis for a multicomponent system, there are only two dyes used in the mixture, the two proportions should be totalled to 1.0. but on finding You got 0.6 in total. Explain the reasons for the difference.5c)Many important bioactive compounds of plants are found in leaves and roots of a plant. Discuss the advantages and limitations of using callus cultures to produce these metabolites.4. When an organism is positive for the coagulase enzyme, which of the following would be present in the test tubes containing rabbit plasma? A. White precipitate at the bottom B. Gel plug C. Liquid D. Pellicle growth at the top
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- 2. Gamma-hemolysis on a blood agar plate would likely appear: Select one: a. As no change in the medium b. Blue c. Yellow d. Green e. As a clearing.It has been noticed that adulteration of palm oil with carcinogenic dyes are on the rise. Use your knowledge in analytical processes to systematically outline a protocol you will follow to process adulterated palm oil to gain qualitative and quantitative information spectroscopicall about any added toxic dye. Explain also the basis for your protocol of choice7. If a patient normally has a 12 g/dL Hb, cyanosis will likely appear when A. 10 g/dL Hb is saturated with oxygen B. 9 g/dL Hb is saturated with oxygen C. 8 g/dL Hb is saturated with oxygen D. 7 g/dL Hb is saturated with oxygen