Procedure Explant Preparation and Inoculation
1) The leaf explants were cut into squares (approximate size = 1cm x 1cm ).
2) The explants were soaked in 95% EtOH for 3 minutes with constant shaking.
3) EtOH was then decanted and 10% of Chlorox bleach was added together with a few drops of Tween 20, with constant shaking.
4) The explants were rinsed with autoclaved distilled water for at least 5 times.
5) The explants were then left to dry in the laminar air flow cabinet.
6) The explants were cultured in the appropriate media (2 explants in basal MS without BAP and the other two in MS with BAP).
7) Three explants were placed on its upper surface down and the other three with lower surface down in both basal MS media with and without BAP.
8) All the culture jars (name, date and treatments employed etc.)were labelled and incubated
them (in dark) in the growth chamber or culture room.
9) Explants were placed on the surface of the medium and the Petri dish were sealed with parafilm.
10) The cultures were observed at weekly intervals for 4 weeks. The changes in the morphology of the cultures and the presence of any infections were recorded.
Based on the picture write description changes in the morphology of the cultures and the presence of any infections.
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