ctively respiring bacterial culture is supplied with acetyl-CoA labeled on the methyl carbon with carbon- 14 (carbon-14 is radioactive). Shortly after adding the label the citric acid intermediates are isolated and the position of the carbon-14 atom is determined. 12A Where is the carbon-14 label on oxaloacetate after two turns of the citric acid cycle? Consider only the initial incorporation of carbon-14 in the first pass of labeled pyruvate through the citric acid cycle. draw the relevant structures that show how pyruvate is transformed to alpha-ketoglutarate. No mechanisms are required, but provide balanced equations. You do not need to draw the structures of cofactors]

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Question #12 An actively respiring bacterial culture is supplied with acetyl-CoA labeled on the methyl carbon with carbon-
14 (carbon-14 is radioactive). Shortly after adding the label the citric acid intermediates are isolated and the position of the
carbon-14 atom is determined.
12A Where is the carbon-14 label on oxaloacetate after two turns of the citric acid cycle? Consider only the initial
incorporation of carbon-14 in the first pass of labeled pyruvate through the citric acid cycle.
relevant structures that show how pyruvate is transformed to alpha-ketoglutarate. No mechanisms are required, but
provide balanced equations. You do not need to draw the structures of cofactors]
draw the
12B. Suppose both of the acetyl-CoA acetate carbons were carbon-14 labeled. When does the first decarboxylation
producing a radioactive 14CO2 occur?
Transcribed Image Text:Question #12 An actively respiring bacterial culture is supplied with acetyl-CoA labeled on the methyl carbon with carbon- 14 (carbon-14 is radioactive). Shortly after adding the label the citric acid intermediates are isolated and the position of the carbon-14 atom is determined. 12A Where is the carbon-14 label on oxaloacetate after two turns of the citric acid cycle? Consider only the initial incorporation of carbon-14 in the first pass of labeled pyruvate through the citric acid cycle. relevant structures that show how pyruvate is transformed to alpha-ketoglutarate. No mechanisms are required, but provide balanced equations. You do not need to draw the structures of cofactors] draw the 12B. Suppose both of the acetyl-CoA acetate carbons were carbon-14 labeled. When does the first decarboxylation producing a radioactive 14CO2 occur?
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