Cuvette 1 2 3 4 Dye Percent Concentration Dye (MM) 100% 75% 50% 25% T 1 Yellow Dye Red Dye Absorbance Absorbance

Introduction to General, Organic and Biochemistry
11th Edition
ISBN:9781285869759
Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Chapter31: Immunochemistry
Section: Chapter Questions
Problem 31.70P
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Record your absorbance data for the YELLOW and RED dyes below. Calculate the dye concentration in mM for each cuvette using M1V2 = M2v2 . Remember the concentration of stock solutions (100^ % dye) is 0.020 mM(mM= millimolar =mm /L). Data for YELLOW: peak absorbance used on spectrophotometer =420nm Cuvette 1 - 100% dye = 0.680 absorption Cuvette 2 - 75% dye = 0.510 absorption Cuvette 3 - 50% dye = 0.340 absorption Cuvette 4 - 25% dye = 0.170 absorption Data for RED - peak absorption used on spectrophotometer = 500nm Cuvette 1 - 100% dye: 0.621 absorption Cuvette 2 - 75%: 0.466 absorption Cuvette 3 - 50% dye: 0.311 absorption Cuvette 4 - 25% dye: 0.1555 absorption
Cuvette
1
2
3
4
Dye
Percent Concentration
Dye
(MM)
100%
75%
50%
25%
Yellow Dye Red Dye
Absorbance Absorbance
Transcribed Image Text:Cuvette 1 2 3 4 Dye Percent Concentration Dye (MM) 100% 75% 50% 25% Yellow Dye Red Dye Absorbance Absorbance
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