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- A microscope that maintains focus when the objective magnification is increased is calleda. binocular.b. myopic.c. parfocal.d. refractive.e. resolute.Explain differential-interference contrast (DIC) microscopy.You have a microscope equipped with 10 x oculars and 4 objectives, which exhibit the following characteristics (M, NA): A=(4 x/0.13); B=(20 x/0.4); C=(20 x/0.7); D=(40 x/0.65) Tick the right answers: o Objectives B and C are equivalent. o Objective D will allow more detailed observation than objective C. o To see small structures, you should choose objective D rather than C. o Objective D has a lower resolution than objective C. o To observe large objects, it is better to use objective B rather than objective C
- As with standard phase contrast microscopy,differential-interference contrast microscopy depends on differences in the ___________________ of a speciment to create high-contrast images. a. refractive index b. light absorption c. natural fluorescence d. walls and coverings e. natural pigmentationExplain the difference between a transmission electronmicrograph (TEM) and a scanning electron micrograph (SEM)a. When buying a microscope, what features are most important tocheck for?b. What is probably true of a $20 microscope that claims to magnify1,000×?
- It is desirable that the microscope objectives be parfocal because the specimen will be .................. a)blurry due to the changes in the refractive index of the medium b)stay in focus when the light is turned off c)have more contrast and enhanced details d)remain in focus if the magnification is changed e)none of the answer choicesYou have a microscope equipped with 10 x oculars and 4 objectives, which exhibit the following characteristics (M, NA): Tick the right answers:o Objectives B and C are equivalent.o Objective D will allow more detailed observation than objective C.o To see small structures, you should choose objective D rather than C.o Objective D has a lower resolution than objective C.o To observe large objects, it is better to use objective B rather than objective CWhat's the importance of objective and ocular magnification in onserving a specimen under a microscope?
- ive the importance of refractometry in pharmacy.What are the advantages of using super-resolution microscopyversus standard fluorescence microscopy?Contrast how the views differ under high power (40x) and oil immerson (100x) objective lenses in terms of a) magnification b) detail of the cell, c) size of the area being viewed and d) resolution.