ou have a microscope equipped with 10 x oculars and 4 objectives, which exhibit the following characteristics (M, NA): A=(4 x/0.13); B=(20 x/0.4); C=(20 x/0.7); D=(40 x/0.65) Tick the right answers: o Objectives B and C are equivalent. o Objective D will allow more detailed observation than objective C. o To see small structures, you should choose objective D rather than C. o Objective D has a lower resolution than objective C. o To observe large objects, it is better to use objective B rather than objective C
Microscopic examination
The analysis of minute organisms, cellular organization of any biological structure, and composition of body fluids with the help of a microscope is known as microscopic examination. The magnification of specimens or samples under study helps in attaining a clearer picture of it.
Gram Staining
Named after Hans Christian Gram, a Danish bacteriologist, Gram stain is one of the most powerful staining techniques within microbiology. This technique was introduced in 1882 to identify pneumonia-causing organisms. The Gram staining technique uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.
You have a microscope equipped with 10 x oculars and 4 objectives, which exhibit the following characteristics (M, NA): A=(4 x/0.13); B=(20 x/0.4); C=(20 x/0.7); D=(40 x/0.65)
Tick the right answers:
o Objectives B and C are equivalent.
o Objective D will allow more detailed observation than objective C.
o To see small structures, you should choose objective D rather than C.
o Objective D has a lower resolution than objective C.
o To observe large objects, it is better to use objective B rather than objective C
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