David isolated 3 unidentified strains of bacteria from a patient's wound. He wants to determine if any of the bacteria are lysogens (contain prophages). Which method should David attempt first? O plate the unknown bacterial strains and spot known phages on them. If the phage cannot infect, then the bacteria are lysogens. O do a growth curve with the bacteria; lysogens grow slower than bacteria without prophages O induce the lytic cycle using mitomycin C and then attempt to infect the bacteria with a lysate from the induced sample O enrich the bacteria samples overnight with M. smeg, and then perform a plaque streak

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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David isolated 3 unidentified strains of bacteria from a patient's wound. He wants to determine if any of the bacteria are lysogens (contain prophages). Which method should David attempt first?
O plate the unknown bacterial strains and spot known phages on them. If the phage cannot infect, then the bacteria are lysogens.
O do a growth curve with the bacteria; lysogens grow slower than bacteria without prophages
O induce the lytic cycle using mitomycin C and then attempt to infect the bacteria with a lysate from the induced sample
O enrich the bacteria samples overnight with M. smeg, and then perform a plaque streak
Transcribed Image Text:David isolated 3 unidentified strains of bacteria from a patient's wound. He wants to determine if any of the bacteria are lysogens (contain prophages). Which method should David attempt first? O plate the unknown bacterial strains and spot known phages on them. If the phage cannot infect, then the bacteria are lysogens. O do a growth curve with the bacteria; lysogens grow slower than bacteria without prophages O induce the lytic cycle using mitomycin C and then attempt to infect the bacteria with a lysate from the induced sample O enrich the bacteria samples overnight with M. smeg, and then perform a plaque streak
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