Q: What can be observed in the experiment entitled "extracting banana DNA"
A: Fruits like strawberries and bananas are mushy and are good for extraction of DNA. Once these fruits…
Q: Gel electrophoresis can be used for all of the following purposes except. (A) Determining the…
A: Gel electrophoresis is the technique of separating macromolecules like DNA, RNA and protein with the…
Q: A ____________ is required to transfer DNA sequences from one organism to another. reporter gene…
A: A reporter gene is a gene that is used as a selectable marker in gene cloning to identify whether a…
Q: What is southern blot? Understand PCR reaction in detail. What polymerase enzyme is used in PCR…
A: Both southern-blotting and PCR are types of recombinant DNA technology. Recombinant DNA technology…
Q: Explain why a quantitative PCR analysis cannot determine the size of the initial template sequence…
A: The combination of the probe-based and intercalating dye-based methods brings the potential…
Q: DNA extraction from banana is being analyzed
A: The stringy substance that you see is DNA! It has been removed from the millions and millions of…
Q: Explain the significance of satellite DNA in DNA profiling technique
A: Deoxyribonucleic acid or DNA is a polymer composed of two polynucleotide chains that coil around…
Q: make a diagram or schematic representation of Restriction fragment length polymorphism or RFLP
A: Genetics is a part of science worried about the investigation of genes, genetic variety, and…
Q: Describe the composition of a DNA microarray, and explain how it is used.
A: A DNA microarray is a laboratory tool which is used to detect the expression of thousands of genes…
Q: Describe the processes involved in denaturing and renaturing of DNA, and explain what is useful…
A: DNA stands for deoxyribonucleic acid. It is the genetic material of the organisms that transfer from…
Q: Describe how each of the following is used DNA hybridization. Polymerase chain reaction (PCR) .…
A: Answer: MOLECULAR TECHNIQUES : These are the techniques which we perform on the genetic molecules…
Q: Recombinant DNA construction involvesa) Cleaving DNA with restriction endonuclease and joining with…
A: Genetic engineering is the branch of biological science that can input alteration of DNA in an…
Q: The purpose of using a washing soap in DNA extraction experiment is to
A: Washing soap is made of salts of fatty acids which dissolves the plasma membrane by solubilization…
Q: What are DNA probes
A: When two single-stranded DNA or single-stranded RNA molecules are allowed to be together and form…
Q: List and briefly describe the function of 4 proteins used in DNA replication in a E. coli but NOT…
A: The process of copying a DNA molecule to produce its two identical copies is termed as the DNA…
Q: Diagram the process by which restriction enzymes andDNA ligase are used to make recombinant…
A: Recombinant DNA technology allows addition of a eukaryotic gene into a bacterial plasmid vector.…
Q: Explain the rationale behind a DNase I footprinting experiment.
A: Genetic Footprint or DNA profile is the basis of Human Identification Genetics. This verifies the…
Q: Give an example of test or application of recombinant DNA in the field of medicine, pharmaceutical…
A: Recombinant DNA technology refers to the modification in the DNA of an organism to obtain the…
Q: Explain what linkers and adapters are used in recombinant DNA studies.
A: Restriction enzymes can either create sticky ends or blunt ends. Sticky ends are those ends that…
Q: Explain the process of bacterial DNA extraction.
A: DNA extraction DNA extraction is a method of separation and isolation of pure DNA from other…
Q: Using the diagram below describe the significance of DNA sequencing for identification of mutations.
A: DNA sequencing is typically the process of determining the nucleic acid sequence the order of…
Q: What are sticky ends? State their significance in recombination DNA technology.
A: Recombinant DNA Technology is a method for creating artificial DNA by combining genetic resources…
Q: Assume the samples in this diagram of gel electrophoresis are DNA molecules. Why do the samples…
A: Gel electrophoresis is used to separate molecules of different sizes. It uses charged molecules that…
Q: Name four reagents used during a PCR reaction
A: Polymerase chain reaction: It is an in vitro technique that results in the exponential…
Q: Outline the steps by which the polymerase chainreaction (PCR) amplifies a specific region of a…
A: Polymerase chain reaction or PCR technique is used to amplify or make multiple copies of a specific…
Q: What type of probe is used for real-time PCR? Explain howthe level of fluorescence correlates with…
A: The amplification of the DNA (deoxyribonucleic acid) or RNA (ribonucleic acid) can be performed by…
Q: Would it be appropriate to use DNA probes such as VNTR in DNA fingerprinting of an bacteriophage?
A: Any group of Viruses that attach to the bacteria and infect the bacteria is known as bacteriophage.…
Q: Explain why PCR results in the amplification of a specific DNA sequence despite many competing…
A: PCR is a very powerful and practical research tool which uses sequencing and identification DNA…
Q: Compare the possible differences between a eukaryotic protein-encoding gene cloned by PCR and the…
A: Specific mRNAs corresponding to a gene of interest are identified and reverse transcribed to form…
Q: Explain the use of a probe in screening a DNA library
A: Introduction: Probes are the small DNA or RNA strands that are used to label DNA. The labeling of…
Q: Describe the purpose and process of DNA finger printing.
A: DNA fingerprinting is the method of recognizing the genetic makeup of individuals through the…
Q: Compare and contrast the following terms: cDNA and gene Restriction fragment and gene DNA probe and…
A: Gene expression is a process in which the genetic instructions of genes are utilized to manage…
Q: What are the major differences between dna probe and Primer
A: DNA as a genetic material in all the living organisms.
Q: Describe the technique of in situ hybridization. Explain how it can be used to map genes.
A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…
Q: how electrophoresis separates DNA strands. How is a DNA fingerprinting test interpreted?
A: NOTE- " Answering the question by explaining the gel electrophoresis as the step for DNA…
Q: Explain the limitation of DNA microarrays ?
A: DNA microarray is the molecular laboratory technique that helps in studying and measuring the…
Q: In regards to using PCR: Explain why a plasmid is often engineered with tetR and lacZ. What purpose…
A: Plasmids are employed in genetic engineering to amplify, or duplicate, certain genes. Plasmids are…
Q: . Explain how gel electrophoresis is used to separate DNA fragments of different lengths.
A: Gel electrophoresis is used for the separation and analysis of the micromolecules and their…
Q: Describe the method of a PCR technique in which you can amplify fragments randomly
A: PCR ( Polymerase chain reaction ) is widely used method for synthesis of replica/ clone of DNA . For…
Q: The purpose of using soap in the DNA extraction activity using frozen strawberries is to
A: A gene is an area of DNA molecule or polymer of amino acid that encodes function. A chromosome…
Q: Explain how reverse-transcriptase PCR is carried out.
A: The reverse transcriptase-polymerase chain reaction is used to identify the genome of many RNA…
Q: From a single DNA molecule, calculate how many copies would be produced in 12 cycles of pcr
A: PCR(Polymerase Chain Reaction) is defined as a type of amplification technique where it amplifies…
Q: Can you explain how PCR( Polymerase Chain Reaction can detect very) low amounts of DNA?
A: Polymerase Chain Reaction (PCR) is a molecular biology technique that is used to amplify a copy of…
Q: Use a drawing to illustrate the principle of DNA gel electrophoresis. (2 marks) - +
A: Gel electrophoresis is a technique used for the separation of molecules on the basis of size and…
Q: Name the five key tools for accomplishing the tasks of recombinant DNA technology.also mention the…
A: Recombinant DNA technology is a process of joining of DNA molecules of two different species. This…
Describe a method of labeling a DNA probe. Explain how this probe maybe detected after DNA hybridization
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- What type of probe is used for real-time PCR? Explain howthe level of fluorescence correlates with the amount of PCRproduct.What type of probe is used for real-time PCR? Explain howthe level of fluorescence correlates with the level of PCRproduct.Describe how gel electrophoresis is used to analyze DNA
- Describe the nature of recognition sites for restriction enzymes and the nature of the ends of the DNA that are left. Why do we need to run a gel electrophoresis after enzyme digestiondescribe the Illumina/Solexa method of "massively parallel DNA sequencing"Explain why a quantitative PCR analysis cannot determine the size of the initial template sequence in a DNA sample.
- What information can be gathered by using DNA gel electrophoresis? Describe the steps in the process and explain how and why gel electrophoresis can be used to separate DNA fragments.Explain how you would use the blue-white colony screening technique to identify bacteria containing the recombinant plasmidUse a drawing to illustrate the principle of DNA gel electrophoresis. (2 marks)-+