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Describe the color and appearance of a Gram positive and Gram negative bacteria as it is treated at every step of the
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- Explain what happens to Gram-positive and Gram-negative bacterial cells at each step of the Gram-staining procedure.The first step in identifying an organism from a pure working culture is to perform a(n) __________ test. acid-fast staining negative staining endospore staining simple staining Gram stainName all the reagents used in Gram-staining and their roles in the staining procedure.
- List 3 species of bacteria that can spoil milk and indicate how they react to the gram staining procedure.After performing the gram stain you observe your result as gram negative. To help confirm this result what type of method (besides the gram stain) could you perform? Please explain why, the result you would want to observe and the interpretation of the result.Describe how the Gram stain is performed and identify the staining characteristics of Gram(+) and Gram(-)organisms. Be sure to include the lenght of time for each step,if water is used to rinse, and identify which step is most critical to doing a quality stain.
- The decolorizing agent damages the peptidoglycan layer. Why is this important to consider when deciding how long to decolorize a specimen as part of the Gram stain procedure?After performing the gram stain you observe your result as gram negative. To help confirm this result what type of method (besides the gram stain) could you perform? Please explainwhy, the result you would want to observe and the interpretation of the result.