Describe how the Gram stain is performed and identify the staining characteristics of Gram(+) and Gram(-)organisms. Be sure to include the lenght of time for each step,if water is used to rinse, and identify which step is most critical to doing a quality stain.

Describe how the Gram stain is performed and identify the staining characteristics of Gram(+) and Gram(-)organisms. Be sure to include the lenght of time for each step,if water is used to rinse, and identify which step is most critical to doing a quality stain.

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.7: Bacterial Identification And Antibiotic Susceptibility Testing

Problem 2RQ

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Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.

Question

Definition Definition Technique that uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.

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