Describe the Ti plasmid binary vector system used in plant transformation and provide details of how this system can be utilized to protect plants against viruses.
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- Describe the Ti plasmid binary vector system usee in plant transformation and provide details of how this system can be utilized to protect plants against viruses.Outline a PCR-based approach to screen for colonies transformed with recombinant expression plasmid that can be used for protein expression.explain the method of Identifying mutant genes by plasmid librarytransformation
- Compare and discuss the key features of plasmids that are respectively used as bacterial cloning and bacterial expression vectors.Explain the process of the DNA recombination for the production of Golden Rice from conventional white rice.Write in detail about the effects of presence as well as absence of MCS and reporter gene in a plasmid vector
- Write in detail about the effects of presence as well as absence of multiple cloning site and reporter genes in a plasmid vector?Describe the molecular features of a BAC cloning vector. What is theprimary advantage of a BAC vector over a plasmid or viral vector?Briefly explain the role of arabinose in the expression of the GFP gene of the pGLO plasmid.
- Bacterial plasmids often serve as cloning vectors. Describe the essential features of a plasmid vector.In gene mapping using generalized transduction, bacterial genes that are cotransduced are a. far apart on the bacterial chromosome. b. on different bacterial chromosomes. c. close together on the bacterial chromosome. d. on a plasmid.Using illustrations describe the structure of a typical cloning vector and discuss the functions of each element.