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Q: Provide an example of an indirect ELISA. Provide an example of a direct ELISA.
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Q: What is the effect of not adding the antigen to the ELISA reaction?
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Q: What is the purpose of a positive control in an ELISA test?
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. Design an ELISA assay for detecting the presence of anti-HIV antibodies in a patient’s serum sample.
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- Is rapid antibody testing a form of ELISA? Explain your answer. How is an ELISA different from rapid antibody testing? Describe how antibodies are used to detect the presence of a specific disease.Explain the ELISA protocol and the purpose of each step.Briefly describe the principles and give an example of the use of aspecific test using immunoelectrophoresis, Western blot, complementfixation, fluorescent testing (direct and indirect), and immunoassays(direct and indirect ELISA).