Discuss    the    principles    underlying    genetic    linkage    and    recombination,    and    the    use    of     these    in    gene    mapping.

Q: What is structural variation of genomes? Describe the mechanisms that create structural variants,…

A: Structural variations are referred to variation or difference in the structure of chromosome of…

Q: How can the concept ofrecombination frequency beused in genetic mapping?

A: Recombination is a process of exchange of genetic material between two different organisms.…

Q: How is the color of the spot coverted into useful data if data is collected from a Vicrochip DNA…

A: DNA microarray is a chip-based technique where a collection of microscopic DNA spots attached to a…

Q: What is a molecular marker? Give two examples. Discuss why it isgenerally easier to locate and map…

A: Introduction: Molecular markers are specific DNA segments identifiable among others in the whole…

Q: List at least two ways in which computerized analysis oftranscriptomes aids genetic research.

A: Genetic research refers to the study of human deoxyribonucleic acid (DNA) to order to know the genes…

Q: List the three features of a cloning vector and why these elements are needed

A: Cloning vectors act as a vehicle to transfer desired foreign genetic material into a cell. All these…

Q: Draw a figure describing the steps in MANUAL Sangers sequencing not automatic sangers sequencing.…

A: Sanger sequencing results in the formation of extension products of various lengths terminated with…

Q: Compare and contrast homologous recombination and site-specific recombination

A: Homologous recombination is a type of genetic recombination in which the genetic information is…

Q: what is Hardy-Weinberg Equilibrium in the Large Scale Genomic Sequencing? how would you summarize…

A: Introduction : Hardy–Weinberg Equilibrium : In The Absence Of Other Evolutionary Factors, The…

Q: How the amplification of other genomic methods such as optical mapping helps to overcome the…

A: The amplification of other genomic methods like optical mapping  has definitely improved the…

Q: Explain the disadvantages of searching for homologous genes and proteins.

A: Homologous genes or protein are searched by using various bioinformatical tools like BLAST, FASTA,…

Q: Describe step 1: hybridization of template and primer in automated sanger sequencing

A: Introduction  Sanger sequencing is also known as the “chain termination method”. It is a method for…

Q: Describe the possible ethical or biological problems associatedwith eating peanuts from an…

A: Genetic engineering is the branch of biological science that can input alteration of DNA in an…

Q: list and explain two strategic between the shotgun sequences/ assemble strategy for whole genome…

A: Genomic Sequencing is a process where whole genome of an organism is sequenced. With the help of…

Q: Explain the basic techniques used to manipulate genetic material

A: Genetic Material:         A cell or an organism comprises information carrier materials found in the…

Q: Outline the procedure of chromatin immunoprecipitation sequencing, and explain how it was used to…

A: Immunoprecipitation is the process of separating out a specific protein from a group of protein. It…

Q: Discuss the concept of the use of DNA in genome identification.

A: DNA (Deoxyribo nucleic acid) is a double stranded genetic element in all prokaryotic and eukaryotic…

Q: What are ‘Selectable marker’? What is their use in genetic engineering?

A: Genetic engineering is a technique to manipulate the genetic content of an organism resulting in…

Q: Compare and contrast the use of histochemical reporter genes and fluorescent reporter genes — what…

A: Reporter genes can be analyzed by different methods, such as beta glucuronidase (uidA) gene…

Q: Explain what linkers and adapters are used in recombinant DNA studies.

A: Restriction enzymes can either create sticky ends or blunt ends. Sticky ends are those ends that…

Q: Briefly describe the steps or methods for studying genes.  Start with targeting a gene region and…

A: Gel electrophoresis is a strategy for investigating DNA dependent on its size (base-pair length). As…

Q: How can linkage disequilibrium mapping sometimes provide a much higher resolution of gene location…

A: Linkage analysis rely on co inheritance of functional polymorphism, In linkage analysis, there are…

Q: Explain the sequencing-by synthesis (SBS) approach ?

A: One of the well-known states that are known as fundamental topics in Arithmetic is sequence and…

Q: Examine the limitations of pedigree analysis inproviding the information needed for positional…

A: Positional cloning is a method used to determine the gene mutations that lead to the development of…

Q: describe how DNA shuffling and molecular breeding are used to generate hybrid genes and the…

A: DNA shuffling or molecular breeding is an in-vitro molecular method, where mutant genes are created…

Q: How to calculate the possibility of gene？Step by step？any faster way？formula？

A: Lets say the homozygous genes be - SSBB for sharp claws and minty breath     S S B SB SB B SB…

Q: Describe the outcome of a chain-terminator sequencing procedure in which (a) too little ddNTP is…

A: Sanger DNA sequencing is also known as the sequencing process of chain-termination. In addition to…

Q: Explain how gel electrophoresis is used to characterize cloned DNA fragments.

A: Gel electrophoresis is a technique in which macromolecules are run on the gel with the help of an…

Q: Mention the advantages and the challenges of using SNP markers in DNA typing

A: Advantages of using SNP markers in DNA typing: 1. The essential explanation given for thinking about…

Q: Explain how substitution matrices can be used to improve the possibility of identifying related…

A: Protein is a macronutrient that is vital for building muscle mass. It is normally found in creature…

Q: Describe the technique of in situ hybridization. Explain how it can be used to map genes.

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: Define hybridization (sanger sequencing)

A: Sanger sequencing is a method which is used to determine the sequence of nucleotides in DNA. This…

Q: DNA polymorphisms can be used as DNA markers. Describe the differences between SNPs and STR…

A: DNA markers can help link an inherited disease with the responsible gene. the most common types of…

Q: Describe why genetic mapping is useful?

A: Gene is a specific nucleotide sequence in RNA or DNA. It is generally located on a chromosome. In…

Q: Draw a simplified schematic for Heidelberg screening to identify zygotic genes.

A: Zygotic mutants P     z/+ ♀      x      z/+♂ F1    z/z           z/+    z/+   +/+

Q: Describe the methodology behind sanger  sequencing techniques

A: Sanger Sequencing techniques is a method of DNA sequencing. It was first developed by Frederick…

Q: Explain the rationale behind the use of the “bag of worms” phenotype as a way to identify…

A: A mutation refers to an alteration in the genome or genetic material of a living organism’s cells or…

Q: Describe how lon torren method of sequencing works in detail.

A: Thermo Fisher Scientific's Ion Torrent technology introduces a completely new approach to Next…

Q: The best molecular technique to quantify the gene transcripts is (write in fu!!).

A: The Central Dogma concept states that "DNA makes RNA and RNA makes protein". This concept is…

Q: Briefly explain why RNA-seq gives more information about the transcriptome than does microarray…

A: Messenger (m-RNA) ribonucleic acid is known to be transcribed from the DNA molecule, which is the…

Q: Explain the procedures for Random Amplified Polymorphic DNA

A: Random Amplified Polymeric DNA (RAPD) are DNA fragments amplified by PCR using short synthetic…

Q: What are microcarrier and macrocarrier in Gene gun method.

A: Biolistic bombartment (gene gun) is one of the direct gene transfer methods. It physically shoot the…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.