Give a step by step method and diagram of how reversible terminator sequencing works.

Q: Why is genome database and annotation relevant to Genome sequencing?   Are there any examples?

A: Genome: the set of genes. Genome database: it is the collection of all information on the human…

Q: Describe the shotgun method for sequencing an entire genome.

A: Genome or genetic material of a cell is the DNA content present in the nucleus. DNA or…

Q: Which is a limitation of using massively parallel sequencing ?

A: It is a term used to describe the method of high-throughput DNA sequencing to determine the entire…

Q: List at least two ways in which computerized analysis oftranscriptomes aids genetic research.

A: Genetic research refers to the study of human deoxyribonucleic acid (DNA) to order to know the genes…

Q: Explain Two strategies for assembling whole genome sequences.

A: All the genetic information of an organism is known as its genome. Genome assembly is defined as the…

Q: Identify a problem in molecular biology and how to use CRISPR/cas 9 system to solve it.

A: "CRISPR" is "clustered regularly interspaced short palindromic repeat". It's a piece of DNA that…

Q: Describe the methods of site-directed mutagenesis and CRISPR-Cas technology.

A: A molecular process in which some discontinuous change to nucleotide sequences in their genomic DNA…

Q: List the different features of coding?

A: The set of different rules that are used through the living cells for translating information that…

Q: Describe step 1: hybridization of template and primer in automated sanger sequencing

A: Introduction  Sanger sequencing is also known as the “chain termination method”. It is a method for…

Q: What are the key differences between DNA microarrays and protein microarrays, and how they are used…

A: Introduction: A laboratory method used to recognize the expression of thousands of genes…

Q: What is synthetic or artificial genome ?

A: A synthetic genome is an artificially built genome that is formed by processes involving genetic…

Q: Outline four key steps involved in producing a recombinant DNA

A: Answer. Recombinant DNA technology  (RDT) is also known as genetic engineering or cloning) is…

Q: What is the difference between using genome build hg18 and hg19 in Genome-wide SNP arrays?

A: SNP (Single nucleotide protein) array is a type of DNA (deoxyribonucleic acid) microarray which is…

Q: What is exome sequencing ? Why it is important ?

A: The exome has historically been described as the sequence in the genome that includes all exons of…

Q: Explain how DNA microarrays are used in the study ofgenomics.

A: DNA (deoxyribonucleic acid) is double helix, coil structure that is responsible for transfer of…

Q: What is the point of genome sequencing?

A: DNA is the main constituent of the chromosome. It contains all information about protein that forms…

Q: Briefly describe the steps or methods for studying genes.  Start with targeting a gene region and…

A: Gel electrophoresis is a strategy for investigating DNA dependent on its size (base-pair length). As…

Q: What is RNA sequencing ? Why it is important ?

A: The biochemical material that is carried from the preceding generation to the succeeding generation…

Q: what are similarities and differences between chain-termination and reversible terminator…

A: Gene sequencing  aids in the development of gene therapy, a form of treatment that replaces faulty…

Q: Explain the sequencing-by synthesis (SBS) approach ?

A: One of the well-known states that are known as fundamental topics in Arithmetic is sequence and…

Q: Define about Sanger sequencing ?

A: Introduction:- The process of determining the sequence of nucleotide bases (As, Ts, Cs, and Gs) in a…

Q: What are the advantages of Next Generation Sequencing?

A: DNA sequencing is a biochemical method for determining the order of nucleotide bases, A, G, C, and…

Q: Explain what is  Flow cell DNA sequencing ?

A: What is DNA sequencing? It is the process of finding out the order of nucleotides i.e. adenine,…

Q: How to find unknown gene and design primers present using whole genome sequence?

A: Step 1 Whole genome sequencing is the process that regulates the complete deoxyribonucleic acid…

Q: Discuss the advantages of next-generation sequencing technologies.

A: Next generation sequencing technique is also known as second generation sequencing where the DNA…

Q: What is the difference between whole exome and whole genome sequencing?

A:

Q: What is shotgun cloning or shotgun sequencing ?

A: Cloning is the process of creating an identical copy of something. In biology, it refers to the…

Q: How can libraries be used to locate a specific gene of interest?

A: Scientists use genetic engineering methods to create two different types of DNA libraries. These are…

Q: Explain how you would compare the expression of a gene in a cancer cell and a normal cell using…

A: The functions of a gene can be studied by looking at how it expresses in a specific cell and how it…

Q: When is low diversity especially problematic in sequencing?

A: In sequencing micro RNA any method which requires use of in house barcodes may results in giving low…

Q: Describe the technique of in situ hybridization. Explain how it can be used to map genes.

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: What are the main  differences between whole genome sequencing and whole exome sequencing?

A: whole genome sequencing is sequencing the entire genome of the organism, where as whole exome…

Q: Explain the impact of chromosome encoding in Genetic algorithms

A: Alleles are the smallest data units in a chromosome. In hereditary calculation, an encoding capacity…

Q: What is the major difference between the strategies of map based sequencing and shotgun sequencing?

A: DNA sequencing is a process in which sequence(order) of nucleotides in DNA is determined. It…

Q: describe one method for sequencing DNA and a method for determining RNA sequencing

A: It means to determine the primary structure of an unbranched biopolymer.

Q: Explain how to determine whether a given clone contains all or only part of the gene ?

A: Gene cloning is described as the process that includes a relocation of gene of interest and which is…

Q: Draw a simplified schematic for Heidelberg screening to identify zygotic genes.

A: Zygotic mutants P     z/+ ♀      x      z/+♂ F1    z/z           z/+    z/+   +/+

Q: "Whole-Genome Sequencing Is Widely Used for Sequencing and Assembling Entire Genomes". Explain this…

A: Whole genome sequencing It reveals an organism's complete DNA make-up ( allowing us to better…

Q: Detail explanation on next generation sequencing

A: Next-generation sequencing is a combination of methods that have produced much higher throughput…

Q: Describe how lon torren method of sequencing works in detail.

A: Thermo Fisher Scientific's Ion Torrent technology introduces a completely new approach to Next…

Q: Explain why exome sequencing can be almost as valuable as genome sequencing. (Explain in your own…

A: Exome sequencing is one of the molecular tool to analyze the genes encoding for protein in entire…

Q: The best molecular technique to quantify the gene transcripts is (write in fu!!).

A: The Central Dogma concept states that "DNA makes RNA and RNA makes protein". This concept is…

Q: Describe how Ion torren method of sequencing works in detail.

A: Thermo Fisher Scientific's Ion Torrent technology brings a whole new method to Next Generation…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.