Question
Asked Nov 18, 2019
9 views

Discuss the similarities and differences between forward and reverse genetic approaches, and when you would choose to utilize each of the approaches.

 

check_circle

Expert Answer

Step 1

Differences between forward and reverse genetics are as follows:

  • Forward genetics involves the elucidation of the gene responsible for a faulty phenotype. However, the reverse genetics approach involves the elucidation of abnormal phenotype of a known mutation in a gene.
  • In reverse genetics, the function of a gene is analyzed. Whereas in forward genetics, the function is already known.
  • Forward genetics involves the isolation of random mutations that would cause a particular mutant phenotype. In reverse genetics, particular gene sequence is altered or its expression is inhibited to study the impact on its phenotype.

The similarities between both approaches is the insertion of mutations or manipulation of gene expression to study the respective phenotypes and genotypes.

 

Step 2

In the reverse genetics approach, targeted mutations are created in a particular gene using site-directed mutagenesis, RNA (ribonucleic acid) silencing. The overexpression of a gene can be studied using insertion of a transgene.

In the forward genetics approach, random mutations are created using chemical or physical mutagens, insertional mutagenesis using transposons. The mutated organisms are ...

Want to see the full answer?

See Solution

Check out a sample Q&A here.

Want to see this answer and more?

Solutions are written by subject experts who are available 24/7. Questions are typically answered within 1 hour.*

See Solution
*Response times may vary by subject and question.
Tagged in

Science

Biology

Genetics

Related Biology Q&A

Find answers to questions asked by student like you

Show more Q&A add
question_answer

Q: Explain the structure of NMJ and name the steps of muscle stimulation in orde

A: Neuromuscular junction a point between the axon and the muscle fiber. Skeletal muscle contraction is...

question_answer

Q: Which one of the following is NOT a feature of chronic obstructive pulmonary disease (COPD)?   Ques...

A: Chronic Obstructive Pulmonary Disease (COPD) is used to describe progressive lung diseases including...

question_answer

Q: What chromosome rearrangement is thought to be the source of new genes?

A: Chromosome rearrangement is a change in the order of base sequences along one or more chromosomes. I...

question_answer

Q: When you hold a weight at arm's length, you are not doing any thermodynamic work but the muscles sup...

A: Muscle contraction:It is defined as the activation of tension-producing locations within muscle fibe...

question_answer

Q: Name the characteristics of muscles. Name the muscle functions. Name the types of muscles and mentio...

A: As per our honor code we are authorized to answer only one question at a moment. In case you need ou...

question_answer

Q: Using the appropriate osmotic terms (hypertonic, hypotonic, or isotonic) describe what would happen ...

A: Click to see the answer

question_answer

Q: I need to know the answer to how these are sorted. Somehow, my original answer was incorrect (master...

A: Adaptive immunity or the acquired immunity has specialized cells that helps in elimination of pathog...

question_answer

Q: What is meant by the term chromatin remodeling? Describe the importance of this process to transcrip...

A: Chromatin remodeling:It is the chromatin rearrangement from a condensed condition to a transcription...

question_answer

Q: What are the phases displayed?

A: Mitosis is the process of cell division occurs in animal cells by which a distinct mother cell divid...