Asked Nov 18, 2019

Discuss the similarities and differences between forward and reverse genetic approaches, and when you would choose to utilize each of the approaches.



Expert Answer

Step 1

Differences between forward and reverse genetics are as follows:

  • Forward genetics involves the elucidation of the gene responsible for a faulty phenotype. However, the reverse genetics approach involves the elucidation of abnormal phenotype of a known mutation in a gene.
  • In reverse genetics, the function of a gene is analyzed. Whereas in forward genetics, the function is already known.
  • Forward genetics involves the isolation of random mutations that would cause a particular mutant phenotype. In reverse genetics, particular gene sequence is altered or its expression is inhibited to study the impact on its phenotype.

The similarities between both approaches is the insertion of mutations or manipulation of gene expression to study the respective phenotypes and genotypes.


Step 2

In the reverse genetics approach, targeted mutations are created in a particular gene using site-directed mutagenesis, RNA (ribonucleic acid) silencing. The overexpression of a gene can be studied using insertion of a transgene.

In the forward genetics approach, random mutations are created using chemical or physical mutagens, insertional mutagenesis using transposons. The mutated organisms are ...

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