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- 1. What is the significance of Rf values?2. What is the basis for the separation of components of mixtures by paperchromatography?3. Give 3 important applications of chromatography.4. Define or describe each of the following terms: - Chromatography- Development- Solvent front- Stationary phase - Chromatogram- Adsorbent- Mobile phase- RfUse the computed dielectric constants of the eluents to discuss the polarity of the compounds you were able to separate and obtain via column chromatography.Explain in detail the advantages in the use of tandem mass spectrometry by referring to the analysis of aspirin (acetylsalicylic acid) with electrospray mass spectrometry combined with chromatography
- Describe the mechanism that allows analites to be separated in:ion-exchange chromatographyWhat is the principle of the instrumental method of analysis of Kool Aid Chromatography? Describe the components' (allura red and brilliant blue) separation of Kool Aid based on mobile and stationary phases.What are the common components of the buffers used in hydrophobic column chromatography? Explain the purpose of each component.
- What is Ion Chromatography? Then, briefly describe the methodology employed in the study. Emphasize the conditions and parameters involved in the studyQuestion: What is the effect of mobile phase composition on the separation efficiency of a reversed-phase chromatography system for the analysis of complex pharmaceutical mixtures(h) Describe a chromatographic technique that could be suitable for studying the molecular weight distribution of poly(phenylenevinylene) derivatives that are soluble in organic solvents. Explain the physical basis on which separation could occur and suggest how the polymers could be detected after elution.
- In color chromatography of plant pigments, what complications would a dried-out column (solvent level is below the top of the silica) introduce to the elution and isolation of pigments?What are the applications of utilizing molecular/size exclusion chromatography in the analysis of samples?discuss the separation of bisphenol z from phenol on the tlc plate in terms of solubility and bonding