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- Silica gel (stationary phase) can potentially interact with analytes by what intermolecular forces?Discuss the syn-anti isomerization of azobenzene in terms of thin-layer chromatography, and discuss the relation between Rf and the polarity of the isomers.What is the main drawback of using steam distillation in the isolation of volatile oils from its matrix?
- Predict the order of elution for the silica gel adsorption TLC separation of the mixture containing aspirin, acetaminophen and caffeine using methylene chloride solvent. Explain.Describe the mechanism that allows analites to be separated in:ion-exchange chromatographyQuestion: What is the effect of mobile phase composition on the separation efficiency of a reversed-phase chromatography system for the analysis of complex pharmaceutical mixtures
- Of Aspirin, ethanol, acetaminophen, ethyl acetate, hexane, and water which could go undetected during HPLC analysis, but could result in a lower than expected melting point, why are they not detected by HPLC analysis?Give 3 examples for inorganic binders and write their approximate calcination temperatures.Arrange the following compounds in order of decreasing ease of elution from a column of silica gel: (a) 2-octanol; (b) 1,3-dichlorobenzene; (c) tert-butylcyclo hexane; (d) benzoic acid.
- Why is distillation of cyclohexanol using acid catalyst phosphoric acid a better technique to purofy rather than extraction, recrystallization, chromatography, IR spectroscopy?The three TLC plates below show the analysis of the same mixture of two compounds. One plate was developed in 100% DCM, one in 90:10 DCM/MeOH, and one in 80:20 DCM/MeOH. Determine which plate was developed in which solvent.In reversed phase HPLC, how will the retention time of a component of a mixture vary with the polarity of its molecules? Explain your answer