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- A. Basic mechanism of 2d gel electrophoresis techniqes? B. How do we predict the protein structure from DNA sequence?With regard to the 30-nm fiber, a key difference between the solenoidand zigzag models isa. the solenoid model suggests a helical structure.b. the zigzag model suggests a more irregular pattern of nucleosomes.c. the zigzag model does not include nucleosomes.d. Both a and b describe key differences between the models.Explain the two-dimensional gel electrophoresis (2DGE) ?
- How to write the methodology? I have a practical of HOMOGENIZATION AND FREEZE-DRYING, I need to do a lab report, but I don't know how to write the methodology. Here is the introduction: HomogenizationHomogenization is a process that involves breaking apart cells to release theircytoplasm and its contents. When the purpose is to extract organelles, it is frequentlydone in two steps; first, using a blender to break the tissue up, and then with anultrasonic or mechanical tissue disruptor. The organelles are then generally separatedusing differential centrifugation.Freeze-dryingFreeze-drying also known as lyophilization, and it is a dehydration process typicallyused to preserve perishable material or make the material more convenient fortransport. It works by freezing the material and then reducing the surrounding pressureto allow the frozen water in the material to sublimate directly from the solid phase tothe gas phase.Answer all whether True or false for the statements given below with a valid reason. a) Ethidium bromide detects 1 pg of double stranded DNA per band. b) RFPL analysis is used to identify a change in the genetic sequence that occurs at recognition site. c) both pst1 and sma1 produce sticky ends. d) chloroform is used to increase the efficiency of isoamylalcohol in solution .Describe FISH analysis and its application in findingspecific DNA sequences in a chromosome.
- Explain the extended and condensed forms of extracted chromatin that have very different appearances in electron micrographs.a) Estimate the lengths of each of the DNA fragments shown in this gel A:____________ B: ____________ C: ____________ D: ____________ E: ____________ F: ____________ b) Which letter represents the DNA fragment that is the smallest? c) Which DNA fragment is approximately the same length as the lengths of fragments D & C added together? Part 4. Putting It Together 1) Consider the diagram below as well as the given information. This diagram represents a piece of circular DNA which was cut in 4 separate reactions (4 different test tubes, each with some of this DNA in it). One digest was done with AvaI, another with ClaI, a third with EcoRV, and a fourth with ScaI. The locations of the recognition sequences for each restriction enzyme are shown along with the location of that site in bp along the circle (it goes clockwise from position 1). You run an agarose gel with a molecular weight marker in the first lane, the AvaI digest in lane 2, the ClaI digest in lane 3, the…Give typing answer with explanation and conclusion 1. Which one of the following sequences best describes the flow of information from a gene to synthesis of a cellular component? A) RNA → DNA → RNA → protein B) DNA → RNA → protein C) DNA → amino acid → RNA → protein D) DNA → tRNA → mRNA → protein E) protein → RNA → DNA
- Use the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy. If the Nco1/EcoRI double digests produces 3 fragments what are their sizes? 2.5 kb, 250 b and 250 b 3.0 kb 2.0 kb, 500 b and 500 b 3.0 kb, 1.0kb and 1.0 kbUse the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy. What is the smallest fragment size that the NcoI/EcoRI double digest produces?Elaborate on the basic principle you would adopt to isolate an amplified DNA fragment using agarose gel during electrophoresis. In you answer, provide factors that would facilitate migrationof the DNA fragment though the gel.
