Q: Why is plasmid useful for DNA transfer?
A: A plasmid is circular, extrachromosomal, small piece of DNA present in the cytoplasm that is…
Q: DEFINE THE FOLLOWING: 1) restriction enzyme 2) plasmid 3) recombinant DNA
A: Ans 1) Restriction enzymes are enzymes that are isolated from bacteria that can cleave…
Q: Bacterial plasmids often serve as cloning vectors. Describe the essential features of a plasmid…
A: Plasmids are the carriers of the genes that are primarily used in biotechnology. Plasmids are the…
Q: How is Ti plasmid of Agrobacterium tumefaciens modified to convert it into a cloning vector?
A: Agrobacterium tumefaciens is a well-studied species of the gram-negative genus of bacteria, known as…
Q: What functions must a plasmid encode to be self-transmissible?
A: A plasmid that codes for its own mating pair formation (MPF) genes is called self transmissible or…
Q: Explain the steps to transform a plasmid carrying a gene of interest into a competent host cell…
A: The deliberate modification of an organism's genetic information by directly changing its nucleic…
Q: A plasmid provides its host with kanamycin resistance. What will happen to the plasmid if you…
A: Plasmids are defined as extrachromosomal circular genetic elements that are capable of…
Q: The F plasmid encodes which two functions essential for conjugation?
A: Plasmid encodes the Synthesis of F pilus and several other proteins required for Conjugation. Plasma…
Q: you transform bacteria with the pGLO plasmid. What do you need to add to the media to get them to…
A: Green Fluorescent Protein (GFP) is obtained from jelly fish (Aequorea victoria). It has excitation…
Q: The ability of F+ cells, or Hfr cells, to transfer plasmid DNA to an F- cell is properly called:…
A: The transmission of the gene between different organisms is known as gene transfer. Gene transfer…
Q: Discuss TWO (2) common methods you could utilize to transform your recombinant plasmids into the…
A: Recombinant plasmids can be transformed into bacterial cells in common molecular biology…
Q: If HIV was just an enveloped virus, but not a retrovirus, which of the following drugs would likely…
A: Virus captures the host replication machinery and multiplies its genome. This is the foundation of…
Q: Briefly describe two different methods for inserting foreign DNA into plasmids, giving the strengths…
A: The plasmid is typically a small circular deoxyribonucleic acid (DNA) strand in the cytoplasm of the…
Q: plasmid has been modified such that it carries the gene for green fluorescent protein (GFP) under…
A: GFP protein is found in the photoorgans of Aequorea Victoria a jelly fish. It is a 28 kDa protein.…
Q: With reference to the image below, discuss the process and principle involved for…
A: Molecular cloning or cloning is a technique to assemble DNA of two different organisms and directing…
Q: What are Bacterial Plasmid Vectors ?
A:
Q: State the differences between the plasmid in Figure 1 with pBR322 in terms of their sercening…
A: According to the question, we have to state the differences between the plasmid in figure 1 with…
Q: What role did the transposon play in the transfer of resistance?
A:
Q: Describe ,A Hfr bacterium mates with an F- plasmid containing bacterium. What
A: PLASMIDS- It was discovered that maleness in bacteria is determined by a transmissible genetic…
Q: The NDM gene is usually plasmid-bourne. What are the implications of this?
A: NDM1 is an enzyme that is used by the bacteria to make itself resistant to the wide range of beta…
Q: All of the following are true about Restriction endonucleases except fashion
A: DNA fragments are cleaved at particular recognition sites by some enzymes known as Restriction…
Q: In Figure 5-2, in which of the four processes shown can acomplete bacterial genome be transferred…
A: Conjugation, transformation, and transduction are the types of horizontal gene transfer mechanisms…
Q: distinguish between bacterial cells that obtained the plasmid and those that did not
A: Bacteria are single celled organisms with a unique internal structure.
Q: The map of plasmid pUC19 is shown below. The restriction site coordinate is the position of the…
A: Introduction Plasmid Vectors: these are like the artificial vehicle to deliver our gene of insert…
Q: is shown below. Draw a restriction map of a plasmid with the appropriate restriction sites based on…
A: Restriction digestion is the fragmentation(cutting) of DNA on very specific sites (restriction…
Q: Demonstrate a procedure that you could adopt to restrict or digest the TOL plasmid.
A: Plasmids are extrachromosomal, circular, double-stranded, self-replicating DNA. They are naturally…
Q: If a F plasmid does not have the gene encoding TraJ, this F plasmid would
A: Conjugation is a major mechanism for dissemination of antibiotic resistance and virulence among…
Q: Predict the sizes of the plasmid cleavage products generated on digestion of pBR322 and pGLO with…
A: Plasmids are naturally occurring extra chromosomal DNA molecules and are mostly used for cloning…
Q: What is the purpose of including a tube in the phage assay in which only the E. coli culture is…
A: Insects that feed on fecal, dung material and raw sewage are termed as coprophagous insects. These…
Q: Describe the generation of multiple-drug-resistantplasmids
A: Drug inactivation or modification is one mechanism of drug resistance. Changes to the target site.…
Q: Write in detail about the effects of presence as well as absence of MCS and reporter gene in a…
A: MCS (Multiple Cloning Sites ) -- Multiple cloning site is region of D.N.A. with in a plasmid that…
Q: If a vector containing the desired gene X is inserted into a microorganism, for instance,…
A: The purpose of this experiment is to insert a vector containing the gene X into the microorganism…
Q: plasmid cloning vector
A: The term vector refers to the DNA molecules that act as transporting vehicle which carries target…
Q: draw structure of plasmid
A: The plasmid is the extrachromosomal deoxyribonucleic acid (DNA) that is present with the bacterial…
Q: Do the plasmids integrate into the host genome when the AAV is introduced into the patient? Explain.
A: AAV (Adeno-associated viruses) are virus that are usually very small in size and that infect primate…
Q: In the bacterial transformation experiment, what is the primary purpose of using an…
A: Bacteria are Prokaryotic microbes which undergo splitting process and form similar cells. Genetic…
Q: Why are plasmids used for transformation?
A: Transformation is the genetic alteration in a cell by direct uptake and incorporation of exogenous…
Q: What and where is the restriction site of the lacl gene of K. pneumoniae? What restriction enzyme…
A: The lacZ and lacI genes are transcribed from opposite strands in the lac operon of K. pneumoniae.…
Q: a. polymerase b. endonuclease c. plasmids d. ligas
A: The process of using recombinant DNA technology to alter the genetic makeup of an organism is known…
Q: Eco R1 Multiple Clone Site (MCS) AmpR Hind 3 LacZ" Plasmid Lacl Amp = Ampicillin Resistance gene Ori…
A: The small DNA piece that is maintained stably in an organism is called a cloning vector. The foreign…
Q: Explain why rolling circle replication of plasmids is semi-conservative.
A: The process of making the copy of DNA is termed as DNA replication. There are three methods for the…
Q: Why are antibiotic resistance genes usually included in plasmids used for genetic transformation?
A: Bacterial transformation is the process of transferring the genetic material from one bacterium to…
Q: Describe the relevant features of the F factor (plasmid) that allow it to partake in conjugation.
A: F factor can exist as an independent plasmid or integrate into the bacterial cell's genome. It can…
Q: How would you select the host cell that has incorporated the plasmid with the foreign DNA…
A: Vector can be defined as DNA molecules which carries foreign DNA into the host,for the expression…
Q: What is the purpose of ampicillin in the pGLO lab, where is the ampicillin locates, how it can be…
A:
Q: True or False: Plasmids can ONLY be used in transforming bacterial cells.
A: Plasmid is small , circular double stranded DNA that is different from chromosomal DNA of cell.…
Q: What are some potential difficulties in using plasmid vectors and bacterial host cells to produce…
A: Recombinant DNA technology involves integrating DNA molecules from two different species and…
Q: Contrast the use of plasmid vectors with that of BAC orYAC (bacterial or yeast artificial…
A: Cloning vectors are also commonly called as vectors. They play an important part in molecular…
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- Illustrate the effect of temperatures 4, 15, and 25 °C on microbial spoilage of food. Assume that spoilage takes place when APC > 5.0 log CFU/g.AtmosphericCO2 concentrations began their most recent steep increase about:a.5000 B.C.b.1000 B.C.c.1400 A.D.d.1800 A.D.e.1950 A.D.Based on the information provided from the report of the West Virginia Chemical Spill and The Human Impact on the Natural Environment, what are the effects humans can have on bodies of water?
- The table below shows the water quality results of palm oil mill effluent (POME)which you have analyzed in your laboratory. You are given a task to proposemitigation options for the palm oil mill after interpreting the findings. Interpretthe characteristics of POME and its potential effect on the aquatic system to helpyou in designing the mitigation options. Parameters Value Department of Environment (DOE) Limit pH 3.4 - 4.8 5 - 9 Biochemical Oxygen Demand (BOD) (mg/L) 25,000 - 43,750 100 Chemical Oxygen Demand (COD) (mg/L) 80,000 - 100,000 100 Oil and Grease (mg/L) 4,000 - 5,000 10What could thwart microbial bioremediation of a sitethat contains buried nuclear weapons that are leakinguranium?What are two reasons there was concern about the 2014 Elk River Chemical Spill?
- match coloumn a to coloumn b coloumn a coloumn b 16. Secondary parameters for water quality are for determining the efficiency ofthe water treatment process.17. In case of an emergency water problem in the La Trinidad water district, theBaguio City Hall must provide a temporary supply for 72 hours.18. To realize the sustainable development goal target 6.1.1, only laboratoriesaccredited by the DOH shall perform drinking water quality examinations.19. A water testing laboratory performing bacteriologic and chemical analysismust have at least six laboratory personnel.20. A water analysis laboratory was sold to another management last August 1,2021. The new management must notify DOH in writing this substantial changeon or before August 15, 2021. a. true b. falseWhat is the epa clean water act?What is water contamination and it sources
- Select the biosafety level shown in the figure. a. Biosafety Level b. Biosafety Level 2 c. Biosafety Level 3 d. Biosafety Level 4are water bodies natural factor or human factor?What are three contaminants that were introduced to the water and how these were identified in the 2014 Elk River Chemical Spill?