A TAMU undergraduate biochemistry student is tasked with characterizing a new enzyme. After devising an assay, the student does some initial experiments. When initiating the reaction by adding the enzyme to a solution of substrates (but no products) she finds that at the earliest times the change in the [A] is non-linear and increasing and then becomes linear for awhile and then decreases eventually to zero. Explain what is unusual and what various aspects of the time course mostly likely means.
A TAMU undergraduate biochemistry student is tasked with characterizing a new enzyme. After devising an assay, the student does some initial experiments. When initiating the reaction by adding the enzyme to a solution of substrates (but no products) she finds that at the earliest times the change in the [A] is non-linear and increasing and then becomes linear for awhile and then decreases eventually to zero. Explain what is unusual and what various aspects of the time course mostly likely means.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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A TAMU undergraduate biochemistry student is tasked with characterizing a new enzyme. After devising an assay, the student does some initial experiments. When initiating the reaction by adding the enzyme to a solution of substrates (but no products) she finds that at the earliest times the change in the [A] is non-linear and increasing and then becomes linear for awhile and then decreases eventually to zero. Explain what is unusual and what various aspects of the time course mostly likely means.
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