The acid fast bacterium, Mycobacterium smegmatis, is relatively safe for students to work with because of its thin cell wall. If a gram stain is to be performed on this particular microbe, it often takes the gram stain reaction. Why?
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The acid fast bacterium, Mycobacterium smegmatis, is relatively safe for students to work with because of its thin cell wall. If a
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- The acid-fast bacterium, Mycobacterium smegmatis, is relatively safe to be used by students in performing the acid-fast stain technique because of its thin cell wall. However, if this organism is used for Gram-stain, it can also take the Gram stain reaction. Why is this so?You accidentally switch the Crystal violet and Safranin steps during your Gram stain. When you look under the microscope, what color will a Gram positive bacteria, like Staphylococcus aureus, be? Red/Pink Purple colorlessConsidering you can’t identify bacteria from a Gram stain, why might a physician perform a Gram stain on a sample before prescribing an antibiotic?
- Bacillus subtilis (Gram-positive) and Pseudomonas aeroginosa (Gram-negative) bacteria are heat-fixed on slide and subjected to Gram staining. What do you observe under the microscope when the following steps are skipped? Please explain.a. Staining with safraninb. Staining with Gram iodinec. DecolorizationYou accidentally switch the Crystal violet and Safranin steps during your Gram stain. When you look under the microscope, what color will a Gram positive bacteria, like Staphylococcus aureus, be? a)red/pink b)purple c)colorlessIn Gram staining method, list the reagents and state their role for each step of the procedure? In preparing bacterial smear, why do we need to pass the slide over the flame?
- You are performing a Gram stain on Gram-negative bacteria, and you stop after the addition of the alcohol. What is the appearance of the bacteria at this point? ○ pink ○ colorless ○ blue ○ purpleA sample must contain many microbial cells for any to be seen by microscopic examination. Why?When doing a gram stain in microbiology, one step can be eliminated and still allow distinction between gram-positive and gram-negative organisms. What is the step and why would eliminating it still result in the ability to distinguish gram-positive from gram-negative?
- Why do you suppose endospore-forming bacteria are commonly found in the soil? Suppose your unknown bacteria is a Gram-negative rod. You decide to perform an endospore stain, thinking it will help you with the identification of the organism. What will you see, and why?Explain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a dayThe decolorizing agent damages the peptidoglycan layer. Why is this important to consider when deciding how long to decolorize a specimen as part of the Gram stain procedure?