What is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What cells will display this stain once you finish the stain and view your slide?

Q: Explain the reasons you would use a negative stain?

A: In a negative stain the slide is actually stained. This is because the negatively charged stain…

Q: What kind of stain would a pathologist use to visualise a chrondrosarcoma under the microscope? why…

A: Staining is a method for enhancing contrast in material, usually on a tiny scale. Biological…

Q: Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram…

A: Gram staining is the common procedure followed to distinguish the bacteria based on their cell wall.…

Q: What are the sources of error in performing Gram staining?

A: Bacteria can be divided into two categories namely gram positive(with thin layer of peptidoglycan)…

Q: Crystal violet is an example of what type of stain?

A: A stain is used to clearly view a specimen under the microscope. For staining, a smear of the…

Q: Why must heat or a surface-active agent be used with application of the primary stain during…

A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…

Q: Explain the mechanism of staining for each of the stains used. Iodine Methyl green Nigrosin

A: The living cells can be seen under a microscope. The cells is not visible properly as it is…

Q: What particular features of fluorescence make it useful for microscopy?

A: Step 1 Microscopes are an instrument which is used for observing small objects and finer internal…

Q: What is the principle application of negative staining?

A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…

Q: Why are basic dyes more effective for bacterial staining than acidic dyes?

A: Staining is a technique used in slide preparation to colour the specimen.      With light microscopy…

Q: For a stain to be considered differential, what must it do?

A: Differential stain uses more than two stains.

Q: Is the flagellar stain a Positive or Negative Simple Stain protocol?

A: Staining is a technique that is used to enhance contrast in samples, generally at the microscopic…

Q: Is heat fixation used on bacterial smears that will undergo a capsule stain or flagella stain…

A: In capsule staining procedure “we do not heat fix and rinse the smear with water” as heat and water…

Q: Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of…

A: Simple staining: Simple stain uses only one stain for staining bacterial cells. It gives basic…

Q: Which bacterial morphology is demonstrated by this simple stain? choose as many as are correct

A: For all types of bacterial cells, a simple staining method may be used to give contrast to the…

Q: What stain would you use to differentiate between bean and ant cells? Explain and include your…

A: Staining is a procedure that involves the application of colored chemicals called dyes to specimens.…

Q: Imagine that you are Gram staining a mixed culture of both gram-positive and gram-negative bacteria.…

A: The bacteria cell envelope is a complex multilayered structure and based on this structure the…

Q: What is the difference between simple stains anddifferential stains?

A: Simple stains :  It generally make all of the organisms in a sample appear to be the same color even…

Q: Why is it sometimes difficult to attain both good resolution and good contrast at the same time for…

A: Magnification and resolution are important because the magnification in larges the image and the…

Q: What are the most commonly used methods in staining? why?

A: Introduction  Staining is a technique for enhancing contrast in material, usually on a tiny scale.…

Q: How are negative stains prepared?Name two applications for which this type of stain especially…

A: Negative staining is defined as a method of demonstrating the type of small objects (like bacteria)…

Q: What are the functions of a primary stain and a counterstain?

A: The main stain and the counterstain must be of different colours so that the two forms of bacterial…

Q: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…

A: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…

Q: What is the advantage of the Kinyoun staining procedure over the Ziehl-Neelsen method?

A: Kinyoun staining is a technique used to stain bacteria. Specifically, acid-fast species of the…

Q: Is acid fast staining differential stain , if yes, what are the 2 types of stain used

A: Differential Staining is defined as a staining process which utilizes more than one chemical stain.…

Q: With what kind of gram stain do you use heat fixation? What is the purpose of heat fixation? What…

A: Gram stain Gram staining is a method of staining which is used to classify the bacterial species…

Q: What is an example of a negative stain?

A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…

Q: two different

A: Gram staining- A test that checks for bacteria at the site of a suspected infection such as the…

Q: What's the theory and conclusion of a acid fast stain?

A: It was Ziehl who originally devised differential staining procedures, that were later refined by…

Q: Which type of stain would be the best choice for negative staining of most bacterial cells: a basic…

A: Gram staining is a technique which is used to identify the bacterial cell type, whether it is gram…

Q: Differentiate the “hot method” and “cold method” of acid-fast staining

A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…

Q: Why don't you heatfix a negative stain slide?

A: Negative staining is used to investigate the physical structure, size, and organization of…

Q: Which type of stain would be the best choice for negative staining of most bacterial cells: a basic…

A: The Gram stain is the most generally utilized staining methodology in bacteriology. This is an…

Q: In negative staining, the acidic stain will penetrate the cell. In negative staining, it will create…

A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…

Q: The primary stain used in the endospore stain is:    A. Carbolfuchsin Stain B. Methylene  Blue C.…

A: To differentiate between the vegetative cells and the endospores, a differential staining technique…

Q: What major advantage does phase-contrast microscopy haveover staining?

A: Phase-contrast microscopy is an optical microscopy technique used in cells. When the light passes…

Q: List the step-by-step procedure of Gram staining.

A: Gram staining, established by Danish Microbiologist Hans Christian Gram in 1884, is by far the most…

Q: Which of the following is/are true regarding the acid-fast stain? (There may be more than one…

A: Answer  First option  Non-acid-fast microbes appear blue in a completed acid-fast stain.

Q: What macromolecules does DAPI stain? What are its advantages and disadvantages?

A: Genetics is the branch of biology that deals with genetic material like DNA, RNA, inheritance.…

Q: What color are Gram negative cells after decolorizing with ethanol? What is the best age for your…

A: Since you have posted a question with multiple sub-parts, we will solve the first three sub-parts…

Q: Describe the procedure for performing a Gram stain. ▪ What is the procedure? ▪ How does each…

A: Bacteria are ubiquitous microscopic , one celled organism which possess variety of shape ,…

Q: k there are 2 staining reagents applied in differential s

A: Differential Staining-  It is a staining process which uses more than one staining reagent.  it is…

Q: What is acid fast staining and why do we use gram staining more?

A: Staining is a method in which images of specimens enhanced at microscopic level. It is commonly used…

Q: What is the type of gram stain?

A: Gram stain or Gram staining, also called Gram's method, is a method of staining used to classify…

Q: Why is methylene blue used to stain cells? What does it bind to?

A: Answer: Methyl blue is used to stain the cell because it is basic dye which can easily bind with…

Q: In acid fast staining, lipoidal material of acid fast cells absorbs the methylene blue. Upon the…

A:

Q: What is the primary stain in the Kinyoun acid-fast stain? How does this differ from the primary…

A: Need to explain the primary stain in the Kinyoun acid-fast stain.

Q: What are the principle and basic concepts of GRAM STAIN?

A: Gram staining is among the most important microbiology staining procedures. It was first invented in…

Q: What is the difference between simple and differential stains? Give an example of a differential…

A: Bacteria are microorganisms that are colorless for most of the part and in order to study their…