DRAW IT A bacterial culture was in log phase in the following figure. At time x, an antibacterial compound was added to the culture. Draw the lines indicating addition of a bactericidal compound and a bacteriostatic compound. Explain why the viable count does not immediately drop to zero at x. Time Log10 of number of cells
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- An inoculum of 106 bacterial cells was introduced into a flask of culture medium and growth monitored. No change was seen for 18 minutes (the lag phase), then growth occurred rapidly. After a further 87 minutes, the population had increased to 5.08 × 107 cells. What is the doubling time of the culture? Show your solution. (The 87-minute period is after the lag phase.)A culture of E. coli has a concentration of 5 x 108cells/mL. How many times do you have todilute the culture so that when you spread 0.1mL on an agar plate you will have 250 colonies?Hint: you need to find dtotal and then convert it into a DF.A culture initially has P0 number of bacteria. At t = 1 hour the number of bacteria is measured to be 3/2P0. If the rate of growth is proportional to the number of bacteria P(t) present at time t,determine the time necessary for the number of bacteria to triple.
- procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (1) broth culture to brothAssume an inoculum with a cell density of 108 cells per mL. The entire generation time takes 30 minutes. How many hours would it take to grow a culture to 108/mL if you started with a 10–2 dilution? helpful formula: g (generation time) = 0.301 (time)/ log x – log xoCiting the data from your results table tell which antibiotic is the most effective against this type of bacterium. Citing the data from the results table tell which antibiotic is the least effective against this type of bacterium. Explain how you determined the level of effectiveness. Describe what the Petri dish looked like. Hint(the most effective antibiotic has a larger clear area around the antibiotic disk. The least effective antibiotic has a smaller clear area around the antibiotic disk.
- Citing data from the results table, tell which antibiotic is most effective against this type of bacterium. Citing data from the results table, which antibiotic if least effective against this type of bacterium. Explain how you determined the level of effectiveness. Describe what that Petri dish looked like. Hint(a most effective antibiotic has a larger clear area around the antibiotic disk. (a least effective antibiotic has a smaller clear area around antibiotic disk.)The figure attached shows the colonies on Eosin Methylene Blue Agar (EMBA) plate. Make a description on the picture.You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies.a) What was the dilution factor?b) How many bacteria were present in the soil?2. Staphylococcus aureus divides every 20 minutes. A culture begins with 10 bacterial cells.a) After 5 hours, how many generations have occurredb) After 5 hours, how many bacteria are present?3. How many milliliters would you need to prepare a 10-2 dilution from a 10ml starting culture?
- When grown in a batch culture, a Bacillus sp. produces an antibiotic only during the stationary phase. Identify the trend of antibiotic productivity in a steady state if it is cultured in a 5 L continuous culture. Justify your response.There are 10 mL of a culture of a bacteria at 104 cells/mL into 990 mL of the same broth at the same temperature (37° C) It is 8 p.m. the night before your lab which starts at 11 a.m. Doubling time for this species is 40 minutes. Will the culture reach Stationary Phase before your lab? Set up the equation correctly, solve for t, and tell me what time the culture will reach Stationary Phase. t /g = (Log Nt – Log N0) /0.301A pure culture was inoculated onto a Mueller-Hinton agar plate. The Kirby-Bauer procedure was performed. One of the drugs tested showed a large zone of inhibition but also had small colonies growing within this zone. Further testing showed that these colonies were not the results of contamination. Why would these colonies be present within this zone of inhibition?