How would you grow a streptomycin-resistant strain E. coli bacteria was spread on both agar plates and incubated for 24 hours. Pos. Srertomycih Neg. Srettomycir If you wanted to grow up a large quantity of streptomycin resistant E. coli, what would you do next?
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Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
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- Based on your disc susceptibility results for E. coli R-, which ONE of the following is correct if considering treatment of an infection caused by this organism? Select one: A. All drugs evaluated could be considered for treatment in this case B. Zone sizes were too large so the results are inconclusive and should be repeated C. Ciprofloxacin is the most potent drug so this should be used preferentially over all others D. Nitrofurantoin and fosfomycin are the most potent so either drug should be used preferentially over all others E. High drug potency is associated with increased toxicity so the drug with the lowest potency should be considered preferentiallyYou have a mixed culture of 2 different bacterial species. One of the species is Gram negative bacilli and the other species is Gram positive cocci. You have noticed that one species is killed by penicillin antibiotics (attacks peptidoglycan) while the other survives. Neither species has antibiotic resistance genes. How can you explain this result? Remember to make direct reference to each bacterial species in your responseKatelyn had been working for Dr. Johnson for a month, and while she had become quite good at measuring inhibitionzones, she didn’t know why she was doing all this work. She had gotten very curious after she began doing all themeasurements on a new set of antibiotics. # is experiment involved infecting mice with MRSA and tracking how theMRSA grew over time.Data were collected by counting the cells of MRSA taken from $ uid samples from the mice. # e cells were measuredby taking one gram of the $ uid and spreading it over plates, but now Katelyn counted the colonies that grew on theplate after 24 hours. Because there were so many, she actually measured the colonies as “log CFU/g.” A CFU is acolony forming unit, or essentially a cell that will divide into a colony that can be seen. Because there can be so many,Katelyn measured them on a logarithmic (log) scale. # e raw data in her lab notebook looked like the following:Table 1. E% ect of treatment on MRSA in mice after 24 hours of drug…
- A researcher wants to compare the pathogenicity of a mutant pathogen relative to wild type in an animal model. The mutant is marked with the constitutive expression of a foc gene that turns colonies blue on X-gal agar. The input ratio of the experiment Dilue/white colonies) was 10:1. The output ratio of the infection experiment tant to d type was 1-100 a. What is the CI? Show your work. 1. Define the Cr and describe what it measures in your answer Which of these genotypes does better during infection, the mutant or the wild-type?About becterial enzymes, it is CORRECT to affirm that: a). Some becterial enzymes can help the microbe fight the immune system, by destroying lgA.b). Coagulases are enzymes that use the host's fibrinogen and form a semi-solid get that isolates amd protects the bacteria.c). Some bacterial enzymes such as hyaluronidase can help the microble move through the host's tissus by destroying the tissue's components.d). Fibrolysin is an enzymes that can help from blood clots.You seed another culture of different end cell line, Walker12 cells, at 8 am on Friday with 10^4 cells. By Thursday at noon (12:00 pm) they reach confluence. Assume the sae confluency as stated above for a T150 flask (culture area of 150cm^2 adn when confluent contains 2 x 10^7 cells). What is the generatoin time (doubling time)? A. 120 hours B. 80 hours C. 38 hours D. 8 hours E. 11.4 hours F. other ________
- What is the relationship between normal flora Escherichia coli (E. coli) and STEC? A When normal flora E.coli divide in two, they sometimes turn into STEC. B Everyone is colonized by STEC but only some people have non-STEC microbes in the large intestine. C Normal flora E.coli do not make Shiga toxin, but STEC are E. coli that do make Shiga toxin.During this outbreak, some people sickened with listeriosis testedpositive for Listeria monocytogenes in their bloodstream, but a DNAprofile of the bacterium showed that it was not the outbreak strain.What likely conclusion can be drawn from this information?a. It is a false positive. b. The person is not part of the outbreak.c. Listeria monocytogenes mutates frequently, making DNAprofiling unreliable.d. The person has already begun to recover from the disease.You perform a Kirby-Bauer assay with two antibiotics. Antibiotic X has a zone of inhibition of 9 mm. Antibiotic Y has a zone of inhibition of 11 mm. Which antibiotic is better at killing this particular microorganism? Group of answer choices 1Antibiotic Y 2Antibiotic X and Y, which have identical antimicrobial activities 3Antibiotic X4 4It is impossible to tell from the information given
- A patient has bloody diarrehea and takes left over penicllin in a medicne cabinet. The culprit causing the infection turns out to be E. coli of a hemorrahgic variety that is Penicllin resistant. Why is the patient is worse condition now?Which of the following statement about normal flora is INCORRECT? Question 3 options: A On Sabouraud's agar, yeast will appear as small creamy colonies and mold will have large fuzzy colonies B Alpha and gamma hemolysis is produced by Streptococcus species C Normal flora never cause disease D A healthy person can carry pathogens like MRSA and Proprionibacterium acnes on our skin E The growth of S. aureus turns the color of MSA to yellowObserve the results from a disc-diffusion experiment below. Which antibiotic would you prescribe to a patient with a similar Staphylococci infection? The patient has no known allergies or contraindications for broad-spectrum antibiotics. Remember to use the table above to determine if the bacteria are resistant, susceptible or intermediate. a. Tetracycline b. Streptomycin c. Penicillin d. Norfloxacin