When grown in a batch culture, a Bacillus sp. produces an antibiotic only during the stationary phase. Identify the trend of antibiotic productivity in a steady state if it is cultured in a 5 L continuous culture. Justify your response.
Q: t /g = (Log Nt – Log N0) /0.301 I introduce a loopful of Escherichia coli cells (say, 1000) into…
A: Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: Discuss why microorganism presents in the nature environment has a longer lag phase and shorter…
A: The microorganisms present in the environment are continually replicating and utilizing the…
Q: A medium containing an abundance of substrate was seeded with 20 mg/L VSS of an active mixed…
A: Biological culture is a common laboratory method for the study of living things. There are different…
Q: Irue or False: An isolated bacterial colony supplies cells to grow a "pure culture" of the bacter…
A: A pure culture of bacteria is a population of bacterial cells growing in the absence of other…
Q: Using one of the graphs obtained with the medium II, name the different phases of the bacterial…
A: Micro organisms are typically grown in a closed culture media. Nutrients are added according to the…
Q: Students in a microbiology lab are provided with a culture of E. coli, and don't know how long it's…
A: Bacterial growth is increase in the quantity of cellular constituents and number .It depends upon…
Q: DRAW IT Draw the following growth curves for E. coli, starting with 100 cells with a generation time…
A: Here, the growth curve will be drawn by plotting Time on X-axis and growth on Y-axis.
Q: Discuss the importance of some bacteria to undergo starvation during stationary phase and importance…
A: The importance of some bacteria to undergo starvation during stationary phase is that : In…
Q: You then take a cotton swab and subculture a sample from this zone of inhibition (where no growth…
A: The Kirby-Bauer test, known as the disk-diffusion method, is the most widely used antibiotic…
Q: The thermal death point is best described as:(a) The highest temperature that will support growth of…
A: BASIC INFORMATION MICROBES They are the unicellular organisms which can not seen by naked eyes…
Q: Draw the following growth curves for E. coli, starting with 100 cells with a generation time of 30…
A: The number of living cells in a bacterial population over time is represented by the bacterial…
Q: In growing E.coli, why is that (reasons) they do not grow after doubling time under 20 degrees…
A: In a core typical range of its growth temperatures (20 to 37°C), Escherichia coli cells will grow…
Q: The phase (s) in a growth curve where bacterial numbers do not increase considerably in a nutrient…
A: In microbiology, the bacterial growth curve is a very essential tool to determine the development of…
Q: The number of bacteria in a culture increases rapidly. The table below gives the number N (t) of…
A: Given Values: Number of bacteria at a time (t=0) = 1000 Number of bacteria at a time (t=3.1 hours) =…
Q: in 5 hours, your bacterial culture went from a concentration of ~105 CFU/mL to ~1.6 x 106 CFU/mL,…
A: n=1.6x106 CFU/ml N=(no of cells at the beginning )=105 CFU/ml n=Nx2x x=no. of generation…
Q: The generation time of a bacterium was measured at two different temperatures. Which results would…
A: The small microscopic, unicellular organisms that are generally not visible to the naked eye are…
Q: Explain the purpose and phase where cells lose flagella and motility in biofilm formation, and why…
A: There are several steps associated with the biofilm formation. The first step includes adherence of…
Q: According to the Liebig's Law of Minimum, which condition of certain growth factor limits growth of…
A: you have asked many questions and according to our instructions we are allowed to answer only three.…
Q: Escherichia coli but not Pyrolobus fumarii will grow at 40°C,while P. fumarii but not E. coli will…
A: Escherichia coli is a Gram-negative, rod-shaped, facultative anaerobic bacteria. It usually inhabits…
Q: In the antibiotic disc diffusion study, the "Minimal Inhibitory Concentration" or MIC of the…
A: Antibiotic disc diffusion study is also known as the Kirby-Bauer method in which the…
Q: Consider the given bacterial growth curve (X axis is time, Y axis is log number of viable cells). At…
A: The number of live cells in a bacterial population over time is represented by the bacterial growth…
Q: In which phase would you expect to find the most endospores in a bacillus cell culture? pick one…
A: Logistic curve: a sigmoid curve that grows exponentially for small values of the variable and…
Q: A fastidious organism must be grown on what type of medium?a. general-purpose mediumb. differential…
A: Bacteria require appropriate environmental conditions for their growth. Solid or a liquid…
Q: In August 2021, there was a multi-state outbreak of melioidosis, an infectious disease caused by the…
A: In microbiology, there have different types of culture mediums that are commonly used to grow…
Q: A loopful of bacteria containing 1000 bacterial cells is inoculated into a nutrient broth and…
A: It is a multiple choice question.
Q: Selective media: O Is regular nutrient agar that can grow any microorganism Selects for the growth…
A: Cultured bacteria are any bacteria that grow in the lab by the microbiologist using the required or…
Q: Describe the difference between the appearance of surface and subsurface colonies in a pour plate.…
A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…
Q: The graph shows the number of microbial cells (log scale) against time in a liquid culture. In which…
A: Bacterial growth is divided into four stages. During the lag phase, the bacterium prepares itself by…
Q: If the temperature of the incubator were to be increased from 35 to 45°C, how would this affect the…
A: The growth of bacteria is influenced by many physical factors. Temperature is one of the significant…
Q: Growth of an E. coli bacteria in batch culture is observed. The bacteria are fed with 0.5 g / L…
A: Bacteria are microorganisms that grow very fast. They almost double every 20 to 30 minutes.…
Q: Media that prohibits the growth of some types of bacteria, but has no reaction to bacterial growth…
A: Introduction Microbiological media, often known as bacterial culture media, is a bacterial growth…
Q: Why would cells that are vigorously growing when inoculated into fresh culture medium have a shorter…
A: Growth curves are generally the description of the density of cell populations in liquid culture…
Q: A medium was inoculated with 5 * 106 cells/ml of Escherichiacoli cells. Following a 1-h lag, the…
A: Growth curves are generally the description of the density of cell populations in liquid culture…
Q: how many hours will it take to produce 10^-6 grams of biomass?
A: Bacterial growth refers to the bacteria proliferation involving the division of cells into…
Q: From the growth OD data alplot a growth curve graph with standard deviation bars blcalculate the…
A: Abstract Error bars commonly appear in figures in publications, but experimental biologists are…
Q: Which B. megaterium culture, a culture in the exponential growth phase or a culture in the…
A: Ultraviolet radiation is a short-wavelength, non-ionizing radiation, and its wavelength ranges from…
Q: Compare and contrast bacteriocidal, bacteriostatic and bacteriolytic agents. What are their effects…
A: It is critical that we understand microbes and their applications since they may be both useful and…
Q: A new intestinal isolate of E. coli is known to have a generation time of 12 minutes. If a culture…
A: generation time is the time required by bacteria to multiply and become double. bacteria has…
Q: Answer the following questions about the graph below Maltose Lactose Time (hours) (i) (ii) (a) What…
A: When Bacteria are grown in a particular culture , there are remarkably various stages of Growth .…
Q: Two bacterial species have generation times of 20 min and 18 hours respectively. From the below…
A: Solution correct answer = Band D B. different species may have very different generation times D.…
Q: use these OD numbers to plot growth curve for E. coli K12– and estimate generation time for this…
A: The growth curve for E.coli K12 is plotted as follows:
Q: Which has a greater impact on the survival of an organism, temperatures above its maximum…
A: The microbes grow in a wide temperature range. The microbial growths are found to be higher at their…
Q: A sharp growth line in the SIM media is an indication of what characteristic? A) Hydrogen sulfide…
A: SIM tests the ability of an organism to do several things: reduce sulfur, produce indole and swim…
Q: If a biotechnologist wishes to obtain a primary metabolite, they will harvest the organism/media…
A: Bacteria are omnipresent and are found in air, water, soil, etc. Each bacterial species has a unique…
Q: s shown in this diagram, you perform a ten-fold serial dilution of a culture to determine the number…
A: CFU count is the technique of enumeration of microbial cells in a sample and it is one of the most…
Q: Bacterial cells are most likely to be affected by antibiotics when they are in this phase of the…
A: Bacterial growth is an orderly increase in the quantity of cellular constituents (e. cell mass) and…
Q: In a new batch of culture medium, a group of sub-lethally wounded bacterial cells were transferred.…
A: Lag Phase- In this phase bacteria are still adapting themselves to the culture and are maturing with…
Q: A bacterium is inoculated into a medium containing two carbon sources, one of which is the…
A: If a bacterium is grown in a medium containing lactose and glucose, it will first utilize glucose…
When grown in a batch culture, a Bacillus sp. produces an antibiotic only during the
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
- A bacterium is inoculated into a medium containing two carbon sources, one of which is the bacterium’s preferred carbon source. The resulting growth curve shows two lag phases. Explain this phenomenon. What is this type of growth called? Include a diagram of what the growth curve would look like and indicate the usage of each carbon source.Starting with 10 bacterial cells per milliliter in a sufficient amount of complete culture medium with a 1-hour lag phase and a 30-minute generation time, how many cells will there be in a liter of medium at the end of 2 hours? At the end of 7 hours? Show your solution.Assume an inoculum with a cell density of 108 cells per mL. The entire generation time takes 30 minutes. How many hours would it take to grow a culture to 108/mL if you started with a 10–2 dilution? helpful formula: g (generation time) = 0.301 (time)/ log x – log xo
- An attempt to transfer bacteria into new media during the death (Decline) phase of a culture resulted in actual growth of the organisms. What is the most likely explanation of this phenomenon?Is the mannitol salt agar (MSA) a complex or defined medium? Explain based on Composition. What kind of media based on what kind of microorganisms it allows to growIf the temperature of the incubator were to be increased from 35 to 45°C, how would this affect the bacterial growth curve of E. coli? (optimum temperature 20o-40oC; most human pathogens)
- Name and explain the culture media that can be used for cultivating non-specific and specific microorganism? b. can culture medium be placed in a freezer? why or why notBacterial cells are most likely to be affected by antibiotics when they are in this phase of the bacterial growth curve.If in 5 hours, your bacterial culture went from a concentration of ~105 CFU/mL to ~1.6 x 106 CFU/mL, what is the best estimate of the doubling time of that species in those culture conditions (based on these numbers)? A. ~1 hour B. ~75 minutes C. ~150 minutes D. ~8 hours E. ~2 hours
- A pure culture of an unknown bacterium was streaked onto plates of a variety of media. You notice that the colony morphology is strikingly different on plates of minimal media with glucose compared to that seen on trypticase soy agar plates. How can you explain these differences in colony morphology?t /g = (Log Nt – Log N0) /0.301 I introduce a loopful of Escherichia coli cells (say, 1000) into 10 mL of Nutrient Broth at 8 p.m. the night before your lab. The cells were taken from a culture plate (Nutrient Agar) held at 37°C, and inoculated into broth at the same temperature. They were held at 37°C overnight in a shaking water bath. At what time would the culture reach the Stationary Phase? Recall that doubling time under optimal conditions (these are) is 20 minutes. A growing bacterial culture has 10,000 CFU/mL at noon and 10,000,000 CFU/mL at 6 p.m. What is the generation time under these conditions? What are your assumptions? At midnight you inoculate 10 mL of a culture of Enterococcus with 103 cells/mL into 990 mL of the same medium, held under the same conditions as the original culture. At what time would the culture reach 107 cells/mL? Assume exponential growth over the period. Assume that g=half an hour. Note: We worked a different variant of this problem in…The data below were obtained for the growth of a pure culture of Escherichia coli in nutrient broth at a temperature of 37°C. Determine (1) the specific growth rate and (2) generation time of E. coli and the duration of the (3) lag and (4) log (or exponential) phases. (ln 2 = 0.693) Time (h) 0 1 2 3 4 8 16 32 Bacterial No./mL 104.1 103.9 104.4 105.5 106.5 107.7 108.0 107.6