The activity provides you with a version of the NGF pathway, an example of a RTK. All of the proteinsinvolved can be in ON/OFF states. Using this cell signaling pathway you will be asked a variety of questions todetermine how the molecules are regulated, but also how a cell would respond if this cell signaling party stepswere interrupted or activated.Neural GrowthFactir (NGF)Ras-Ras-GDPGTPTrk-ARas-GEFbrafbrafМЕКМЕКERKERKERKERKSTATSTATSpiouty DNANucleusCytosol Erk and P-Erk live in two different locations within the cell even though they are just two different conformations ofthe same protein (one shape for Erk and another shape for P-Erk. A) What are those two locations? B) Explain whatother protein would be needed to allow this protein to move between locations (think protein sorting lectures).

* Mitogen activated protein kinase also called as MAPK and p42MAPK, and ERK2 * It is an enzyme…

Erk and P-Erk live in two different locations within the cell even though they are just two different conformations of the same protein (one shape for Erk and another shape for P-Erk. A) What are those two locations? B) Explain what other protein would be needed to allow this protein to move between locations (think protein sorting lectures).

Erk and P-Erk live in two different locations within the cell even though they are just two different conformations of the same protein (one shape for Erk and another shape for P-Erk. A) What are those two locations? B) Explain what other protein would be needed to allow this protein to move between locations (think protein sorting lectures).

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter9: Cell Communication

Section: Chapter Questions

Problem 1VCQ: Figure 9.8 HER2 is a receptor tyrosine kinase. In 30 percent of human breast cancers, HER2 is...

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A scientist observes a mutation in the transmembrane region of EGFR that eliminates its ability to be stabilized by binding interactions during dimerization after ligand binding. Which hypothesis regarding the effect of this mutation on EGF signaling is most likely to be correct? EGF signaling cascades would be active for longer in the cell. EGF signaling cascades would be active for a shorter period of time in the cell. EGF signaling cascades would not occur. EGF signaling would be unaffected.
During signal transduction (a) the cell converts an extracellular signal into an intracellular signal that leads to a change in some cell process (b) a signaling molecule directly activates or represses several genes (c) each enzyme catalyzes production of one molecule of product (d) enzymes in the signal cascade remain active until the last component of the pathway alters a cellular process (e) the signal is terminated by cyclic AMP
Figure 9.8 HER2 is a receptor tyrosine kinase. In 30 percent of human breast cancers, HER2 is permanently activated, resulting in unregulated cell division. Lapatinib, a drug used to treat breast cancer, inhibits HER2 receptor tyrosine kinase autophosphorylation (the process by which the receptor adds phosphates onto itself), thus reducing tumor growth by 50 percent. Besides autophosphorylation, which of the following steps would be inhibited by Lapatinib? Signaling molecule binding, dimerization, and the downstream cellular response. Dimerization, and the downstream cellular response. The downstream cellular response. Phosphatase activity, dimerization, and the downsteam cellular response.
. Hedgehog, the signal, does not travel very far to bind to its receptor, Patched, on target cells.Therefore, target cells that are closer to the signaling cell receive a stronger signal. What modeof signaling does this describe?A. EndocrineB. Paracrine/AutocrineC. SynapticD. Contact-dependentExplain:
a universal feature of cell signaling receptors is that they? a. undergo a structural (shape) change when the signal molecule is bound b. are present only in the plasma membrane c. are only ion channels d. are protein kinases e. aer only nuclear receptors
A scientist observes a mutation in the transmembrane region of EGFR that eliminates its ability to be stabilized by binding interactions during dimerization after ligand binding. Which hypothesis regarding the effect of this mutation on EGF signaling is most likely to be correct? a. EGF signaling cascades would be active for longer in the cell. b. EGF signaling cascades would be active for a shorter period of time in the cell. c. EGF signaling cascades would not occur. d. EGF signaling would be unaffected.
Illustrate a complete cell signaling cascade from signal to response. Each protein in the pathways must be shown. You can choose either a GPCR or an RTK mediated pathway.
EGF signals by binding to cell surface EGF receptors. Which of these observations, if true, would BEST explain EGF’s mechanism of action? A. EGF is hydrophilic and can easily diffuse through the cell membrane B. EGF is hydrophobic and can easily diffuse through the cell membrane C. EGF is hydrophilic and cannot diffuse through the cell membrane D. EGF is hydrophobic and cannot diffuse through the cell membrane
Even in the presence of a Ras-GAP, a single amino acid change in as renders it incapable of hydrolyzing GTP. This mutation is known as Ras+ and is a cancer-causing mutation. What effect do you think this mutation will have on signaling downstream of Ras+? Why? a)A mutation would turn on the signaling pathway all of the time. b)Even if a route is mutated, it can still be turned on or off. c)Due to a mutation, the signaling pathway would always be off.
When epidermal growth factor (EGF)—the ligand for the epidermal growth factor receptor (EGFR)—binds EGFR on the cell surface, the intracellular domain of the receptor changes shape into an "active state.” The receptor then induces a cascade of kinases in the cytoplasm, resulting in the activation of mitogen-activated protein kinase (MAPK). One effect of EGFR signaling is the stimulation of cell proliferation. Overactive EGFR is frequently found in cancer cells. One strategy for developing anticancer drugs is to target the overactive signaling proteins found in cancer cells. Antibodies are proteins made by immune cells that only recognize specific biomolecules, called antigens. The major component of an anticancer drug is an antibody that specifically recognizes and binds EGFR. Which of the following will most likely happen when this anticancer drug is applied to a tumor that has a high level of EGFR? Tumor cells with active EGFR will increase and MAPK activity in the tumor will…
Receptor, especially growth factor receptor signaling is deregulated in cancer cells, which results in uncontrolled growth. The alterations responsible for this may be due to a truncated receptor, or change from normal paracrine to autocrine signaling or ligand (growth factor) independent firing or overexpression of a receptor Explain how each of these changes results in excessive signaling by the receptor.
Binding EGF to the EGF receptor causes phosphorylation of tyrosines on the cytoplasmic tail of the receptor. Which of the following best describes the mechanism by which this phosphorylation activates downstream signaling complexes? Select one A. Causes degradation of receptor by proteasome pathway B. Causes EGF receptor to be internalized so it can interact directly with downstream signaling molecules C. Tyrosine phosphorylation alters 3D structure of downstream signaling proteins causing them to change from an inactive to active conformation D. Causes release of EGF from receptor E. Alters the localization of downstream signaling partners in the cytoplasm