Experiment 1.2 - Reaction over time 2. Using the standard curve in 1.1, calculate the amount of product formed (in M) at each time point in the following table, then plot a curve of product formation against reaction time. Absorbance at 415 nm Cuvette 2+ (PNPP + alkaline phosphatase) 04 0.404 0.716 1 t t t t له t t t t t 1 t t t Time (min) 90 80 70 60 50 40 30 20 10 0 0 10 ܢ.15 20 25+ 30€ 35+ 40€ 50+ 60+ 70€ 80+ 90€ 0 10 0.936 1.066+ 1.1374 1.179 1.203 1.215 1.224 1.228 1.230 1.232 1.234 20 30 40 [PNP] (UM) Cuvette 2+ (PNPP + alkaline phosphatase) 0+ 24.871 45+ 59.194 67.581 72.161 74.871 50 76.419 77.194 77.774 78.032 78.161 78.290€ 78.419 PNP 60 70 ✔ A ✔ e e ✔ A 80 90 100
Experiment 1.2 - Reaction over time 2. Using the standard curve in 1.1, calculate the amount of product formed (in M) at each time point in the following table, then plot a curve of product formation against reaction time. Absorbance at 415 nm Cuvette 2+ (PNPP + alkaline phosphatase) 04 0.404 0.716 1 t t t t له t t t t t 1 t t t Time (min) 90 80 70 60 50 40 30 20 10 0 0 10 ܢ.15 20 25+ 30€ 35+ 40€ 50+ 60+ 70€ 80+ 90€ 0 10 0.936 1.066+ 1.1374 1.179 1.203 1.215 1.224 1.228 1.230 1.232 1.234 20 30 40 [PNP] (UM) Cuvette 2+ (PNPP + alkaline phosphatase) 0+ 24.871 45+ 59.194 67.581 72.161 74.871 50 76.419 77.194 77.774 78.032 78.161 78.290€ 78.419 PNP 60 70 ✔ A ✔ e e ✔ A 80 90 100
Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter46: Gas Exchange: The Respiratory System
Section: Chapter Questions
Problem 14TYK
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