Explain how biotechnology and bioinformatics are used to identify microorganisms Explain why biotechnology has replaced many of the traditional diagnostic tools in microbiology Explain why a PCR reaction needs a source of nucleotides in the form of ATP, GTP, CTP and TTP. Explain what primers are and describe their use in PCR reactions

Explain how biotechnology and bioinformatics are used to identify microorganisms Explain why biotechnology has replaced many of the traditional diagnostic tools in microbiology Explain why a PCR reaction needs a source of nucleotides in the form of ATP, GTP, CTP and TTP. Explain what primers are and describe their use in PCR reactions

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 17QP

See similar textbooks

Similar questions

PCR has revolutionalized biotechnology. Discuss three advantages and three disadvantages of PCR usage.
When [ATP] is high and in excess, phosphocreatine (PCr) is made as an energy reservoir in muscle and brain that require bursts of energy. Creatine (Cr) + ATP-4 ⇄ PCr-2 + ADP-3 + H+ (near equilibrium) When a sudden demand for energy depletes ATP, the PCr reservoir is used to replenish ATP. One may ask why the body does not store ATP at higher concentrations and rather store excessive energy in the form of PCr. Since many enzymes are activated or inhibited by higher [ATP], excessive ATP cannot be stored inside of the cells. Looking at the above reaction equation, discuss an advantage of using PCr as the energy reservoir
What are the different types of PCR and their product concepts? Site and explain the comparison briefly.
PCR is a powerful technique to screen and amplify segments of DNA for use in recombinant protein technologies. Describe in detail, the components of a PCR reaction and why they are required
Explain why a positive control and negative control are included in PCR experiments. Explain the three steps involved in each cycle of polymerase chain reaction.Why is loading dye added to the DNA sample for gel electrophoresis? Explain the function of the following components in a PCR reaction:− Primer, dNTP, MgCl, Taq polymerase, buffer.
Quantitative PCR (qPCR) has one extra ingredient to quantify DNA. Describe its structure and explain how it changes during the reaction.
Quantitative PCR differs from regular PCR in that it uses [A] to [B] the amount of [C] in a sample. It cannot quantify [D] unless it is first made into [F]. Match each of the following to its appropriate letter: quantify, cDNA, RNA, DNA or RNA, fluorescence. 1) A 2) B 3) C 4) D 5) E Here are the choices for the questions a) quantify b) RNA c) cDNA d) fluorescence e) DNA or RNA
Cite three examples of an application of PCR (polymerase chain reaction).
Before Taq polymerase or Pfu polymerase were available, scientists used regular E. coli polymerase. Explain what was the hassle involved in using this polymerase for PCR, and then why the Taq or Pfu polymerase offered a solution to this problem. (Clearly address both parts of this question.)
Why is it important to sequence positive clones derived from PCR cloning? Group of answer choices Errors could have been incorporated during restriction enzyme digestion and so it is important to verify that only the expected protein is produced. Errors could have been incorporated during plasmid DNA extraction and so it is important to verify that only the expected protein is produced. Errors could have been incorporated during PCR and so it is important to verify that only the expected protein is produced. Errors could have been incorporated during cloning and so it is important to verify that only the expected protein is produced.
Briefly Explain the role of restriction enzymes in recombinant DNA technology. Please explain at your own words.
indicate which of the following statements is/are FALSE for qPCR A. The sequence order of steps for a qPCR is 1. Denature 2. Anneal 3. Detect 4. Extend B. qPCR estimates the quantity of template nucleic acid according to the change in fluorescence during the reaction. C. The estimation of nucleic acid quantity is performed at the end point after ~30 -35 PCR cycles D. The absorbance at 260 nm is used in qPCR to estimate the initial amount of DNA in the reaction. E. Intercalating dyes are added to the qPCR to estimate the quantity of dsDNA F. The fluorescent probes used in qPCR are extended by the polymerase