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- Explain how methyl-directed mismatch repair candistinguish which strand to repair when replicationerrors occur.Explain whether mismatch repair reduce the replication error if it is repaired without regard for which strand served as template.Explain why base excision repair, nucleotide excision repair, and mismatch repair—which all require nucleases to excise damaged DNA—require DNA ligase.
- For each DNA repair process in column I, list all characteristics fromcolumn II that correctly describe that process.I(a) Nucleotide excision repair(b) Photoreactivation(c) Base excision repair(d) Recombinational repair(e) SOS-driven error-prone repair(f) Alkyltransferase repair(g) Mismatch repair(h) Double-strand break repairII1. RecA protein participates.2. Damaged nucleotides are removed by nick translation.3. A free radical mechanism is involved.4. The repair enzyme functions only once.5. The key enzyme contains a bound folate cofactor.6. No bases or nucleotides are removed from the DNA.7. Deficiency of this enzyme in humans greatly increases the risk of skincancer.8. This system is chiefly responsible for the mutagenic effect of ultravioletlight.9. This process begins with cleavage of two phosphodiester bonds.10. This process begins up to 1 kbp away from the site to be repaired.11. DNA ligase catalyzes the final reaction.12. This process also occurs in meiotic recombination.13.…Discuss the similarities and differences between nucleotide excisionrepair and the mismatch repair system.Explain why a DSB-repair homologous recombination can occur between any two DNA molecules that share homology rather than only between two DNA molecules that carry a specific sequence.
- 2b) Suppose you generated a mutant E. coli strain in which it was DNA Polymerase I that was inactivated - assuming that all the other enzymes involved in replication remained fully functional, how would DNA replication in these mutant cells lacking DNA Pol I differ from DNA replication in normal E. coli? Briefly explain why you would expect to see that change/those changes in DNA replication in the mutant cells.Bloom syndrome in regards to faulty DNA give detailed reponses provide exmaplesHow does SeqA prevent DnaA–ATP from binding to the oriCregions immediately after chromosome replication initiates?