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Explain how to conduct a Kirby-Bauer disk diffusion test. Include all of the steps involved.
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- What is the purpose of Biuret’s test? In biuret’s test, does the color in each test tube containing the sample vary? Why or why not?DILUTION COLONY COUNT CFU/mL 1:106 155000 1:107 15500 1:108 1550 1:109 155 Given these values how would I fill in the rest of this serial dilution table? Also, what would be a 1:1 CFU/mL value based on this table?For a laboratory exercise of antibiotic susceptibility test in a disk-diffusion method. What are the concentrations of antibiotic applied in the test?
- What does an ‘I’ result mean in disk diffusion susceptibility test? answer is intermediate please help to explainWhy would it be important for the Kirby-Bauer disc diffusion test to use a standard concentration of the bacterial strain being tested?Discuss the medical application of the Benedict’s test? What other test(s) are used in parallel to Benedict’s test?
- If you plate 200 ul of a 1:100,000 dilution and get 123 colonies, what is the number of CFU/mL in the original sample?If the 1-mL sample had been added to 99 mL of diluent to make the first dilution (instead of 9 mL), how many cells would be in that particular dilution?how does the diameter of the column affect the flow rate of the elution in gel filtration chromatography?