how does the diameter of the column affect the flow rate of the elution in gel filtration chromatography?

Q: What are the expected problems in the centrifuge in case of imbalance of samples ? *

A: Centrifugation is a process of separation of molecules from mixtures by applying centrifugal force…

Q: You aseptically transfer 1 mL of your original liquid culture into 99 mL of sterile water. What is…

A: The dilution factor is a term used to describe the ratio of the final volume over the aliquot…

Q: What is the pore size of the membrane filter used in the membrane filter method for water analysis?

A: Microorganisms are small organism that cannot be seen by naked eyes. Infectious disease is caused by…

Q: What are the pros and cons of serial dilution method and W/V % method?

A:

Q: How to count the number of colonies forming units per milliliter (CFUs per ml) of the sample?

A: A viable cell is one that has the ability to divide and form a colony. The colony-forming unit (CFU)…

Q: where will you dispose of the waste from Molisch test?

A: Sugars or carbohydrates are the most abundant biopolymers in living organisms. Carbohydrates are…

Q: Before inoculating and pouring molten nutrient agar into a plate, why must the agar first be cooled…

A: Agar or agar-agar is a jelly-like substances obtained from red algae. Agar is a solidifying agent…

Q: gel filtration chromatography using a column experiment.

A: Introduction  Gel-filtration chromatography is a type of parcel chromatography used to isolate…

Q: What will you do if you see bubbles forming on the gel after pouring it onto the gel caster/tray?

A: In laboratory, Electrophoresis is a technique for separation of the proteins and nucleic acid. The…

Q: Why is the annealing temperature different between samples? How is this computed?

A: Introduction Polymerase chain reaction or PCR  laboratory technique by which millions to billions of…

Q: During cell couting, when the data are plotted in an ordinary graph or on a cross-section paper, why…

A: For experiments involving growth of cells whether bacterial or eukaryotic the results of a growth…

Q: In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored?

A: Gel-filtration chromatography is a type of partition chromatography that is used to separate…

Q: How do you calculate the initial concentration of a sample? How would you make a 1:10 dilution of a…

A: Hi! As you have posted multiple questions and have not mentioned which is to be answered, we are…

Q: What are the advantages and disadvantages of the filter method in the analysis of water samples?

A: Utilising the membrane filter method, test is gone through the layer utilising a filter funnel and…

Q: Why is Endo Agar is recommended for membrane filtration experiment and not TSA plate?

A: Membrane filtration is a water sample testing technique. Water is drawn through a specific porous…

Q: what is the effect and rationale when three milliliters instead of 1 mL was placed in the Petri dish…

A: Pour-plate method is usually the method of choice for counting the number of colony-forming bacteria…

Q: What are seven applications of gel filtration

A: Gel filtration is a biochemical technique by which molecules such as proteins or small molecules are…

Q: Why did the blank used in experimental procedures contain substrate, buffer and water?

A: A blank or blank determination is an analysis without a sample (Enzyme). Any enzyme assay requires a…

Q: Describe in detail the process of electrospray ionization ?

A: Ionisation is done by high voltage. Ions spread evenly on a surface. Ionised liquid gives rise to…

Q: How does gel filtration work? What are the applications of analytical versus preparative versions…

A: Chromatography is a technique for separating a mixture in the laboratory. The mixture is dissolved…

Q: You count 4 squares of a hemacytrometer and ontain 82,65,85 cells in each squy.what is the cell…

A: Hemocytometer is device used for the counting of blood cells in a sample. It has counting chambers…

Q: What is the concentration of a 50% solution after completing three 1:10 serial dilutions?

A: Serial dilution is the process that reduces the concentration of a solution in an organised manner.…

Q: Gel-filtration chromatography separates molecules according to their size . Smaller molecules…

A: Gel-filtration chromatography is a type of separation technique that uses a gel as a medium through…

Q: Label the void volume (V0), elution volume (Ve) and total elution volume (Vt) appropriately on the…

A: By the gel filtration chromatography the protein or other biological molecules of solutions…

Q: calculate the CFU/ml of a urine sample if 84 E. coli colonies were counted on NA when 50 microliters…

A: Serial dilution is the process of diluting a sample serially by taking the aliquot of one dilution…

Q: How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the…

A: Serial dilution is used mostly in studies involving cell cultures (microbiology or even cell culture…

Q: What is the basic principle of GPC (gel permeation chromatography)?

A: Gel permeation chromatography is a type of size-exclusion chromatography that uses organic solvents…

Q: Briefly explain the principle behind the following techniques: (a) ion-exchange chromatography  (b)…

A: Chromatography is a biophysical method for separating compounds from a mixture. It works on the…

Q: What is the purpose of the sodium thiosulphate in the testing of treated water?

A: Testing of wastewater and treated water both demand a standardized process to be followed to obtain…

Q: What is electroporation technique?

A:

Q: In acid-fast determination, what is the method of collection in comatose or bedridden patients?

A: Acid fast staining is basically a microbiological  test which helps to detect presence of acid fast…

Q: Why is the incubation temperature criticalwhen using the Filter Method when testing water samples?

A: Incubation temperature is critical for bacterial growth if this temperature is too high or too low…

Q: briefly explain the principle behind this technique; gel-filtration chromatography

A: First of all, let's discuss gel-filtration chromatography. This technique is also known as the size…

Q: Using absorbance readings and a standard curve relating absorbance to cell number, you suspect that…

A: The countable range of colonies for an ideal plate count method is considered 30-300 colonies per…

Q: What is meant by the term plating efficiency?

A: The cells are cultured over the plate at the particular growth conditions within a particular time…

Q: Please calculate the denaturation percentage

A: Biomolecules are the biological molecules that are present inside the living organisms. These…

Q: mportance of pH adjustment in culture media?

A: Culture media is also known as growth media. It is a mixture of nutrients that helps for the growth…

Q: To measure the culture temperature, why is the thermometer placed in a tube separate from the…

A: A microbe or microorganism is a microscopic organism that is too small to be seen with the naked…

Q: With the aid of a diagram, explain the principles of gel filtration chromatography.

A: Gel filtration chromatography: Chromatography is an analytical technique where the components of a…

Q: Why does Alka-Seltzer foam and bubble when dissolved in water? Which ingredient is the antacid?

A: Alka- Seltzar is a type of medicine which is used to cure indigestion, stomach pain or upset…

Q: In making red blood cell suspension, why do we need to wash red blood cells in saline?

A: Red cell suspension is a common reagent used for many serologic procedures. Red cell suspensions…

Q: How much liquid media should you put in your dilution blank if you wanted to serially dilute a1 ml…

A: Serial dilution of the original sample containing microorganisms to achieve countable colonies on…

Q: You dilute 1 mL of bacterial culture with 99 mL of water.  What is the resulting dilution factor?

A: Dilution factor:In order to calculate the Dilution factor, the final volume is to be divided by the…

Q: What is the order of elution of proteins on a gel-filtration column? Why is this so? What are two…

A: Different chromatographic techniques have different methods of purification of substances. It is…

Q: You are asked to add 2 mL of bacterial culture to 98 mL of water. What is your dilution factor? Show…

A: Introduction :- A test to determine whether you have a bacterial infection is a bacterium culture.…

Q: how to calculate the experiment fow rate for this gel chromatography Experiment flow rate ? Flow…

A: Given Values: Total volume eluted out of the gel filtration column = 15 ml The time taken in seconds…

Q: He then ran the sample on an agarose gel and observed a strong band at approximately 400bp. What…

A: The band represent a small piece of DNA that was cut with restriction endonuclease and then…

Q: After a urine culture, 95 colonies are counted on a plate inoculated with 1 uL of urine samplpe.…

A: The CFU count gives us the viable number of cells present in the sample.

Q: What happens if you exclude one or more data sets of the peak area ratio of the chromatograph, how…

A: In chromatography technique, the mixture of components are separated based on their retention times.…

Q: If you have a culture with 1 x 106 cells per ml, how could you use serial dilutions to obtain a…

A: Serial dilution  It is a method of stepwise dilution of a concentrated solution to get the desired…