Adding 1 mL of culture to 9 mL of diluent results in a 1:10 dilution. 1 mL 1 mL 1 mL 1 mL Original bacterial culture to 9 mL diluent to 9 mL diluent to 9 mL diluent to 9 mL diluent 1:10 dilution 1:100 dilution 1:1,000 dilution 1:10,000 dilution 50,000 5,000 500 50 cells/mL cells/mL cells/mL cells/mL cells/mL 1 mL 1 mL 1 mL 1 mL 1 mL Too many cells produce too many colonies to count. Too many cells produce too many colonies to count. Too many cells produce too many colonies to count. Does not produce enough colonies for a valid count. Between 30-300 cells produces a countable plate. FIGURE 4.17 Making Serial Dilutions To decrease the concentration of cells in a sample, 10-fold dilutions are often used.

Adding 1 mL of culture to 9 mL of diluent results in a 1:10 dilution. 1 mL 1 mL 1 mL 1 mL Original bacterial culture to 9 mL diluent to 9 mL diluent to 9 mL diluent to 9 mL diluent 1:10 dilution 1:100 dilution 1:1,000 dilution 1:10,000 dilution 50,000 5,000 500 50 cells/mL cells/mL cells/mL cells/mL cells/mL 1 mL 1 mL 1 mL 1 mL 1 mL Too many cells produce too many colonies to count. Too many cells produce too many colonies to count. Too many cells produce too many colonies to count. Does not produce enough colonies for a valid count. Between 30-300 cells produces a countable plate. FIGURE 4.17 Making Serial Dilutions To decrease the concentration of cells in a sample, 10-fold dilutions are often used.

Principles Of Pharmacology Med Assist

6th Edition

ISBN:9781337512442

Author:RICE

Publisher:RICE

Chapter12: Parenteral Equipment And Supplies

Section: Chapter Questions

Problem 23RQ

See similar textbooks

Similar questions

A laboratory technician performed a series of 10 fold serial dilutions shown below. he plated 0.1 mL from each dilution tube onto NA and got the follow numbers per plate dilution 1:10 1:10 1:100 1:1000 1:10000 1:100000 colony count >1000 >1000 808 303 38 3 calculate the concentration of bacteria in the undiluted stock (in CFU/mL)
In this experiment, a culture was serially diluted to the concentrations below. Each plate was plated with 0.25mL of the dilution. Using the most diluted plate, what is the correct concentration of the original culture? A) 2.0 X 10^-3 cells/mL B) 2.0 X 10^5 cells/mL C) 4.0 X 10^5 cells/mL D) 5.0 X 10^4 cells/mL
Look for two product of Milk in the internet and answer the following queston: 1. Method of sterilization used. 2. What is the Bacterial culture and flavor used if there is any. 3. What is the Fat, Protein & Carbohydrate(sugar) content? 4. Is fortification done? What substances has been fortified (based on the label)? 5. Are Vitamins & amino acids added? Name them based on the label.
In spread plate method, inoculating loop is used to spread bacteria from the inoculation site. For quadrant streaking, the loop must be flame sterilized between quadrant streaks. a. First statement is TRUE, Second statement is FALSE. b. First statement is FALSE, Second statement is TRUE. c. Both statements are TRUE. d. Both statements are FALSE.
A bacterial culture has a concentration of 3.2 x 108 cells /mL. You dilute this culture as follows: 1/50, then 10-3and finally 1/20. If you then plate 0.2 mL of the final dilution, how many CFU would you expect following incubation?
TRUE or FALSE only! 1. Pure cultures contain only a single species and no other organisms. 2. Pour plate method is done by pipetting liquified warm agar into a petri dish containing an inoculum. 3. Flaming the test tube’s mouth is necessary so that the moisture that accumulated will evaporate. 4. After Gram staining, the Gram positive bacteria will appear blue while the Gram negative bacteria will appear red.
The following is result from a standard plate count. What is the cell density of the undiluted culture? Please follow all SPC rules. (TNTC: too numerous to count)
Identification of an organism does not require a pure culture because Bergey’s Manual will sort out the contaminant. Identification of an organism does not require a pure culture because Bergey’s Manual will sort out the contaminant. True False
1 ml of a patient sample was added to 999 ml of media. From this 0.1 ml was transferred to the plate. After 24 hours there were 60 colonies on the plate. What is the dilution factor here. What's the number of bacteria per ml of the original patient sample. a. The dilution factor is 1000. The patient sample contains 600 cells/ml b. The dilution factor is 1000. The patient sample contains 600,000 cells/ml c. The dilution factor is 100. The patient sample contains 600,000 cells/ml d. The dilution factor is 10. The patient sample contains 6000 cells/ml e. It cant be determined its too many cells
3.1 You can perform a specific staining method to confirm the purity on the culture. Describe this staining method. 3.2 Describe the results obtained from 3.1 and comment about the purity of the culture. 3.3 What staining method would you use to verify that the isolate is not producing survivalstructures against unfavourable conditions? Describe the procedure as well.
A plate has 72 colonies with a total dilution factor of 10-7, 0.1 ml was pipetted onto the plate, what was the original CFU/ml concentration of that sample?
You were tasked to prepare four petri plates. Three of the petri dishes were inoculated using different samples. The plates were prepared from the following: Plate A: oral swab after brushing Plate B: cat’s paw Plate C: exposed in air for one hour Plate D: uninoculated The plates were incubated at 37˚C for 24 hours. The following results were obtained: 1. Compare the growth of the cultures found in Plates A, B, C and D. Which among the four plates has shown the most growth? Why do you think so? 2. Describe the appearance and abundance of the colonies found in your four plates. What possible type of microorganisms are found in your plates? 3. Although sparse, there is still visible growth of microorganisms in Plate D (uninoculated plate). Where do you think the microorganism came from? REFERENCES: