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Explain the roles of lipopolysaccharides in gram negative structure and
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- Explain the difference between Gram positive and Gram negative cell walls. Describe the procedure for performing a Gram stain. What is the procedure? How does each component work? (ie. What does the mordant do?) How is the Gram stain used clinically to help identify the cause of an infection?Describe the staining of Acid fast negative organisms.Explain the relationship between bacterial cell wall with gram-positive and gram-negative in the staining process. Please state chemical used and their function.
- Explain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a dayExplain why a green metallic sheen is formed when E. coli is grown on Eosin Methylene Blue Agar.In Microbiology What does a metallic green sheen indicate on an EMB (Eosin Methylene Blue agar)plate? How is this metallic green sheen formed?
- Answer the following questions What is the purpose of the 95% ethanol step in the Gram staining procedure? (1 Mark) xiii) What is the function of the following reagents used in Gram stain: a) Crystal Violet b) Gram’s iodine c) Acetone/alcohol d) Safranin ISeveral students argued about why Gram-positive cells stain purple with the Gram stain whereas Gram-negative cells stain pink. Which explanation is correct? Multiple Choice Alcohol dehydrates peptidoglycan; the thick layer of dehydrated peptidoglycan in Gram-negative cells prevents the crystal violet from being washed out Safranin stains lipopolysaccharide, and because only Gram-negative cells have lipopolysaccharide, those cells look pink. Safranin stains lipopolysaccharide, and because only Gram-negative cells have lipopolysaccharide, those cells look pink. Alcohol dehydrates peptidoglycan; the thick layer of dehydrated peptidoglycan in Gram-positive cells prevents the crystal violet from being washed out. Crystal violet specifically stains peptidoglycan, and because Gram-positive cells have a thicker peptidoglycan layer those cells look purple. Alcohol destroys lipopolysaccharide in Gram-negative cell walls, allowing safranin to enter the cells and staining them pink.Archaea with cell walls consisting of a thick, homogeneous layer of complex polysaccharides often retain crystal violet dye when stained using the Gram-staining procedure. Suggest a reason for this staining reaction.