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Describe the staining of Acid fast positive organisms.
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- Describe the staining of Acid fast negative organisms.Explain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a dayWrite down the materials, reagents, Principle, procedure and results for Mann’s methyl blue-eosin stain
- Write the protocol for lactophenol blue stain method indicating; materials, reagents, Principle, procedure and results.Give the principle of acid fast staining and gram staining.The acid fast technique is also known as: A. Ziehl-Neelsen stain B. Auramine-rhodamine stain C. Gerrard-Lampard Stain D. Kinyoun method
- In Gram staining method, list the reagents and state their role for each step of the procedure? In preparing bacterial smear, why do we need to pass the slide over the flame?ou make a smear of E. coli and then simple stain the slide with methylene blue. If you failed to use correct aseptic technique by not sterilizing the loop before picking up the bacterial sample for the smear, what results may be seen under the microscope?write the protocol for mann's methyl blue-eosin staining, including their reagents, materials, prinicple, procedures results, and cautions
- Enumerate the advantages derived from staining bacteria. In Gram staining method, list the reagents and state their role for each step of the procedure? In preparing bacterial smear, why do we need to pass the slide over the flame?Name the reagents used in Gram-staining and their roles in the staining procedureDistinguish between a sterilant, a disinfectant, and anantiseptic. What is cold sterilization?