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A: The data given is,
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- For a Kjeldahl analysis: you weighed 0.0312 g unknown dried blood sample and digested it with concentrated H2SO4. After the distillation into boric acid solution, Methyl Purple indicator changed its color to green. During titration with 0.0110 M H2SO4 it took 15.35 mL of this H2SO4 solution to bring the color of the mixture to grey-blue. a) What number of moles of H2SO4 reacted? b) What number of moles of boric acid reacted with NH3 during distillation? c) What number of moles of NH3 was produced during distillation? d) What was mass of N in the produced NH3? e) What is %N w/w in the original sample?Mention what error would be introduced in the analysis if the primary standard (KHP) is not properly dried.Need help with Analytical Chemistry. Explain the stepwise production of titratable I2 from dissolved O2 in the sample.
- Approximately 6 mL of concentrated perchloric acid (72%) was transferred to a bottle and diluted with about 1.0 L of water. A sample containing 250.0 mg of primary standard sodium tetraborate (Na2B4O7 .10H2O, FW= 381.42 g/mol) was dissolved in 50.00 mL water and required 27.25 mL of the HClO4 solution to reach the methyl red end point. A 25.00 mL solution of an unknown NH3 sample required 13.24 mL of the HClO4 solution. Calculate the molarity of the NH3? Use the factor label method in answering.Describe why the inside wall of the flask is rinsed with deionized water during accurate titration. Does this washing affect the molar quantity of acetic acid in the sample? Describe..?Which of the following steps would affect both yield and purity during the extraction of ovalbumin?a. Washing the precipitate with ethanol before storageb. Using KCl instead of NaCl for the storage solution c. Adding (NH4)2SO4 in bulk instead of in incrementsd. Making use of a centrifuge with a larger diameter
- Calculate the number of grams of oxalic acid dihydrate (M.W. = 126.1g/mol) necessary to react with 35 mL of the 0.1 M NaOH solution. Showall your calculations. Note: 35 mL is the approximate volume of NaOHsolution that needs to be used in the titration in order to obtain theprecision desired in this experiment.Why is a titrant of very low concentration (0.002 M) used in this experiment? The experiment is the Determination of Vitamin C by Redox Titration.The Kjeldahl procedure was used to analyze 244 µL of a solution containing 38.1 mg protein/mL. The liberated NH3 was collected in 5.00 mL of 0.0375 M HCl and the remaining acid required 5.98 mL of 0.00998 M NaOH for complete titration. What is the weight percent of nitrogen in the protein?
- The Kjeldahl procedure was used to analyze 256 L of a solution containing 37.9 mg protein/mL. The liberated NH3 was collected in 5.00 mL of 0.033 6 M HCl, and the remaining acid required 6.34 mL of 0.010 0 M NaOH for complete titration.2) What’s the principle of extraction and recrystallization ? 2) What’s the best method to separate the compound? Why2. In the preparation of the titrant, the solution must be aged for at least 24 hours in the dark. State in just 1 sentence why this step must be done.