Figure 1. Cytotoxicity test on 96 well plates that was monitored by ELISA reader 3- Discussion 1- Explain another methods by which cytotoxicity is measured ?
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- EXPERIMENT: CRYOPRESERVATION OF CULTURED CELL LINES Procedure for Cryopreserving MCF-7 and THP-1 Cells• Ensure that the culture is up to the late log phase (assumption is made based on the cell concentration). Prepare the cell suspension and determine the cell concentration.• Dilute the cell suspension in 1:1 ratio with freezing medium (final concentration approximately 1 X 106 – 1 X 107cells/ml is desired). • Dispense the cell suspension into prelabeled cryovial and place the vial in -80°C freezer overnight (temporary storage).• Transfer the cryovial into liquid nitrogen cryotank for long term storage the following day.• After day 3, determine the cell concentration in the freezing medium andcompare with that of the stock concentration. Question: Please briefly explain what is cryopreservation(1), how procedure above are useful to make this experiment successful(2)Compare the lysis for bacteria to that for CHO (Chinese Hamster Ovary) cells. Note, CHO cells are commonly used for production of therapeutics. How resistant are CHO cells compared to the bacterial cells? If the same level of disruption is expected for CHO cells and the homogenizer is operated at the same pressure for both types of cells, would you expect CHO cells to require more or less number of passes compared to E.coli or Bacillis brevis cells?Identify the Boundary Condition (2) For each scenario, choose which boundary condition would be appropriate. Consider and list what assumptions you might be making. 5. In a bioreactor, enzymes are bound to glass beads. Substrates diffuse in the media to/from the beads. 6. Oxygen diffuses towards a single cell in a culture dish. It is rapidly consumed at the cell surface. 7. Crystals of active pharmaceutical ingredient (API) are mixed vigorously to dissolve them into a formulation. 8. Dissolved solute flows through a tube.
- (a) Insert the table with data for Experiment 1 Table 1. Osmosis in a model cell contents of dialysis bag contents of beaker mass at 0 min (g) mass at 15 min (g) mass at 30 min (g) mass at 45 min (g) 1 2% sucrose 2% sucrose 11.66 11.75 11.67 11.73 2 20% sucrose 2% sucrose 12.54 12.72 12.98 13.21 3 2% sucrose 20% sucrose 12.11 11.86 11.59 11.48 (b) Calculate the total change in volume with the appropriate units for each dialysis bag in the experiment. Bag 1: Bag 2: Bag 3: (c) Explain why the volume of each bag changed during the experiment.Osmosis is often defined as the flow of water through a semipermeable membrane, from a dilute solution to a more concentrated solution. Video experiment #1 – saturated sucrose/dialysis tubing/water osmosis from U Mich. https://youtu.be/pCupvFGN4bw A saturated solution of sucrose-containing a red dye is separated from water by dialysis material. What happens to the volume of sucrose solution during the experiment? How do you know that the sucrose solution did not flow into the beaker of water? (Give evidence from your observation of the experiment.) Diffusion, Osmosis, Dialysis video: https://youtu.be/tHzkRtzVmUMDialysis, the flow of both solvent and small molecules or ions through a dialyzing membrane, is important clinically in the operation of the artificial kidney machine.Liquid supported membrane of DGA used for rare earth elements separation. Tell me the background/introduction of this research, current state of the art, motivation, how these membrane are made , what is the appropriate characteristics of them, approaches that author followed, you can describe the problems of that approaches, why rare earth is challenging to separate, why current state of the art do not address that need, include two or 3 results and describe it. take those information from few journal papers and mention the correct citation.
- describe the steps involved in primary cell dissociation protocol, assessing cell viability and sub culturing adherent cells with trypsin.33. Which statements concerning passive and active transport are correct? A. Both passive and active transport requires cell energy B. Passive requires cell energy while active transport does not C. Active transport requires cell energy while passive does not D. Neither passive nor active transport requires cell energyExperiment No. 1. Five rabbits were inoculated in the right lung and in the left side of the neck with five minims of sterilized water in which was suspended a sufficient quantity of a pure culture (third generation) of the tubercle bacillus to render the liquid quite perceptibly turbid The needle of the Koch’s inoculating syringe was inserted subcutaneously on the left side of the neck and in the third intercostal space to a depth of thirty millimetres on the right side. These animals were then confined in a small box and put in a dark cellar. They were thus deprived of light, fresh air and exercise and were also stinted in the quantity of food given them while being themselves artificially infected with the tubercle bacillus. Experiment No. 2. Five healthy rabbits were placed under the following conditions: A fresh hole about ten feet deep was dug in the middle of a field, and the animals having been confined in a small box with high sides but no top, were lowered to the bottom of…
- Experiment No. 1. Five rabbits were inoculated in the right lung and in the left side of the neck with five minims of sterilized water in which was suspended a sufficient quantity of a pure culture (third generation) of the tubercle bacillus to render the liquid quite perceptibly turbid The needle of the Koch’s inoculating syringe was inserted subcutaneously on the left side of the neck and in the third intercostal space to a depth of thirty millimetres on the right side. These animals were then confined in a small box and put in a dark cellar. They were thus deprived of light, fresh air and exercise and were also stinted in the quantity of food given them while being themselves artificially infected with the tubercle bacillus. Experiment No. 2. Five healthy rabbits were placed under the following conditions: A fresh hole about ten feet deep was dug in the middle of a field, and the animals having been confined in a small box with high sides but no top, were lowered to the bottom of…Sterile normal saline is used in most surgical procedures as an irrigation solution. During certain surgical procedures, such as those for malignancy, the surgeon may request that sterile water be used for irrigation instead of saline following excision of the cancerous mass. Why is normal saline usually used in healthy tissues?What part of a cell would be considered the hazmat crew ( detoxifies waste) ?