Figure 5: MCF-7 cells were incubated for 24 h with the drug dilution buffer (control) or various concentrations of indibulin. They were then stained with an antibody against α-tubulin (red channel), and Hoechst 33258 (blue channel). Scale bar = 10 μm. Question : Comment Figure 5

Q: This medication should be diluted with Normal Saline. How would you dilute this medication to obtain…

A: The nurse has to follow the 5 Rights while administering the medication such as The Right drug, The…

Q: To determine whether an enzyme-linked antibody is bound to an antigen, __________ must be added,…

A: ELISA is an enzyme linked immunosorbent Assay which is a biochemical test used to detect the…

Q: What does an ‘I’ result mean in disk diffusion susceptibility test?

A: Susceptibility tests determine a microbe's vulnerability to antimicrobial drugs by exposing a…

Q: Principle underlying neutralization tests.

A: Antibodies are specific proteins that are secreted by the body to kill or deactivate the harmful…

Q: Name the tests we used to test for marcomolecules.

A: The names of the tests used to detect macromolecules.

Q: describe the steps involved in primary cell dissociation protocol, assessing cell viability and sub…

A: Answer :- As we know that Cell viability examines give a readout of cell wellbeing through…

Q: What do you mean by ultrafiltration?

A: The excretory system is the system that performs excretion, the bodily process of discharging…

Q: Calculate the quantity of glycerin needed to prepare 35-mL of the following ophthalmic solution.…

A: Dexamethasone sodium phosphate is a salt of dexamethasone that is particularly suitable for…

Q: With ischemia, which of the following occurs in cells? Decreased: 1) sodium diffusion into cells 2)…

A: Ischemia is the reduced blood flow causing a shortage of oxygen in the body. Reduced availability of…

Q: At 10 am you received a doctors order that you have to Infuse 1,000ml of PNSS in 10 hours to Mr.…

A: 3. 16.67 gtts/min

Q: For the following scenario you must draw the situation depicted, indicate which solution is…

A: Osmosis is phenomenon of the movement of solvent molecules from a lesser concentration gradient to a…

Q: a 55 -year-old man who has upon catheterization two-vessel CAD. He has adverse effects on nitrates…

A: CAD stands for coronary artery disease in which the coronary arteries in the heart become narrow.…

Q: Match the expected result (purple, red, or colorless) to the following descriptions of Gram-stained…

A: Gram staining is a common staining technique that is used to differentiate bacteria based on their…

Q: You perform a series of 4 different dilutions of 200 µL of sample into 800 µL of saline. What is the…

A: Dilution factor can be calculated using the formula:C1V1=C2V2

Q: What will happen if the concentration gradient is high inside the cell and low outside the cell?…

A: Plasma membrane is semi permeable and allow the selection of some material to transport across the…

Q: Fill in the blank:  White blood cells whose cytoplasmic granules stain red in acid stain are called…

A: Blood is a body fluid in humans and other animals that delivers necessary substances such as…

Q: Which of the following can not be used to mediate the fusion of plasma membranes of two different…

A: Cell to cell fusion is a highly regulated cellular process in which several uninuclear cells combine…

Q: Given the information provided, please answer the following question: Volume of cell suspension…

A: For setting up the new culture of the cells, the volume of cell suspension required is to be…

Q: Marco is given an immortal cell line of epithelial cells to dilute. The initial concentration is…

A: Serial dilution is a process in which the sample is diluted in a series of a standard volume of…

Q: Intravenous solutions must be prepared so that they are isotonic to red blood cells. A 0.9% salt…

A: The tonicity of a solution is defined as its ability to permit the movement of water molecules…

Q: The third micrograph is at the lowest magnification. Identify A (name of the cells; blank 1).…

A: These micrographs are of the organ :-  Spinal cord

Q: CONCENTRATION (NG/ ML) ABSORBANCE 655 NM (AU) 0.695 0.699 1000 500 250 0.587 125 0.513 63 0.385 31…

A: ELISA stands for enzyme-linked immunosorbent assay. It is a commonly used bio-chemical assay, that…

Q: Standard curves You are setting up a standard curve for lysozyme. You have a stock vial containing 2…

A: Proteins are chains of amino acids bonded via amide bonds. Lysozyme is an antimicrobial enzyme that…

Q: Complete the following:  After comparing the wet mount and the stained cheek cells, state the…

A: Cheek cells are those cells that are normally shed from the mouth lining. These cells usually…

Q: When a red blood cell placed in a ypotonic solution, a red blood cell will lose water and undergo…

A: Tonicity is a measure of the effective osmotic pressure gradient ,the water potential of two…

Q: Intravenous solutions must be prepared, to that they are isotonic to red blood cells. A 0.9% salt…

A: Introduction  Isotonic solutions are those that have the same concentration of water and solutes as…

Q: The cell is usually placed after the monochromator, except in the case of using a multichannel…

A: A multi-channel analyzer is an instrument used in laboratory and field applications so to analyze an…

Q: In less than 10 words, answer the following question.   What would happen if you increased the…

A: MALDI TOF It is a Matrix-assisted laser desorption Ionization-Time of Flight The basic purpose of…

Q: Look up and list the size of the following proteins; then predict in what order they would be eluted…

A: Introduction Size-exclusion chromatography, also known as molecular sieve chromatography, is a…

Q: Two-dimensional normal phase ascending TLC is used for the following reasons: for further separation…

A: Introduction TLC or Two-dimensional chromatography is a type of chromatographic technique which is…

Q: Dosage Calculations Calculate the following dosages. What volume of PCN G is in one dose? Ordered:…

A: Question is related to drug calculation  Solution is given below step

Q: hemocytometer

A: INTRODUCTION : Experiment Aim  is to determine the viable cell concentration . Before getting into…

Q: Label the nucleus, cytoplasm, and cell membrane of the following pictures. The red one is the human…

A: Introduction: Erythrocytes in humans are produced in the bone marrow and the average life span of an…

Q: In the classic 1950s recording by David Rogers , Rogers shows a white blood cell chasing a bacterium…

A: White blood cells are a type of cell that helps to fight infection by engulfing and destroying…

Q: Can a doctor make a plan for treatment for a person based solely on the information in the gel…

A: Gel electrophoresis is a laboratory technique for separating DNA, RNA, and protein mixtures based on…

Q: After 1000 mL of solution is given to a patient via an IV, then 20 mL per minute was given. This…

A: A variable is a characteristic that can be assigned different values. There are two kinds on…

Q: A cell population has 10,000,000 cells and you add a disinfectant. Assume the constant D-value is 5…

A: A cell is the basic structural and functional unit of an organism. A cell population includes a…

Q: What is the purpose of sheath fluid in a flow cytometer?

A: Introduction Flow Cytometry:- It is a technique used to detect and measure the chemical and physical…

Q: Below is shown the FRAP profiles of three proteins X, Y and Z, which of the protein(s) is/are most…

A: FRAP stands for fluoresence recovery after photobeaching(FRAP) is a technique to study mobility of…

Q: Using a semi-permeable membrane, dialysis allows the removal of salt ions prior to chromatography.…

A: Dialysis is a separation process that uses selective and passive diffusion via a semi-permeable…

Q: True or False: A normal red blood cell is labeled in the picture above. The other cell is shriveled…

A: Solution is made by the combination of solute molecules with Solvent. Solution = Solute + solvent…

Q: Explain this assertion using the example of hematoxylin and eosin staining.

A: Hematoxylin and eosin staining is used for identification of diseases like cancer. These are two…

Q: Inflammatory responses are associated with lots of swelling, where fluids rush into cells and…

A: Inflammation is caused by the body’s response to an injury or pathogen attack or some other…

Q: 0.9% salt (NaCl) concentration is isotonic to red blood cells. What would happen to a patient's red…

A: Osmosis is defined as the movement of water molecules from the higher the potential to the lower…

Q: Why is it important to know the isotonic point of human cells when administering an IV? Explain what…

A: Isotonic solutions are IV fluids that have a similar concentration of dissolved particles as blood.…

Q: How can we find the resistance of a cell-membrane?

A: Introduction: Cells are the underlying, practical, and organic units of every single living being. A…

Q: In this experiment 4 dialysis tubes filled with unknown starch concentrations were placed into…

A: Hypertonic Solution:  Hypertonic solution is defined as solution that contains more dissolved…

Q: Short wavelength irradiation (~260 nm) of cells will cause which abnormality? (best answer)

A: Ultraviolet light is the part of the electromagnetic spectrum between 200 and 400 nm, with shorter…

Osmosis

In simple words, osmosis is the slow, gradual movement of a liquid through a membrane. But there are nuances to this process. For instance, there has to be an imbalance in the distribution of solute in the liquid on either side of the membrane, which has to be semipermeable. The liquid in the region with a lower solute concentration moves to the region with a higher concentration, thereby displacing the solution in the process. The objective is to equalize the concentration of molecules on both sides of the membrane; the displacement of liquid can be viewed as a byproduct of this equalization. Since there is no energy required to carry osmosis, it is considered a passive process.