A cell population has 10,000,000 cells and you add a disinfectant. Assume the constant D-value is 5 minutes. How many cells will be left after 10 minutes?
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A cell population has 10,000,000 cells and you add a disinfectant. Assume the constant D-value is 5 minutes. How many cells will be left after 10 minutes?
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- If you started with a bacterial concentration of 2,000,000 bacteria/ml and wanted to plate out 40 bacteria what would be the dilution scheme of using 4 dilutionsYou have a culture of cells at 3.5 x 108 cells/ml and you want to prepare a fresh 5 ml culture with a starting density of 1 x 104 cells/ml. What volume of the old culture do you add to the new 5 ml culture?There are five bacteria in a petri dish and its number triples every minute. How many bacteria are in the petri dish after 10 minutes.?
- When applying a disinfectant to kill microbes, our bead experiment showed that what two factors affect how effectively microbes will be killed?If the D value of a microorganism is known, what else can we find? Explain by showing the relationship between D value and your answer.You place 1 ml of a bacterial broth culture into bottle A containing 99 ml of sterile water and mix. You take 1 ml from bottle A and transfer it to bottle B, which contains 99 ml of sterile water. What is the dilution of the bacterial culture in bottle B?
- If the 1-mL sample had been added to 99 mL of diluent to make the first dilution (instead of 9 mL), how many cells would be in that particular dilution?How can organisms develop resistance to chemical disinfectants?List chemical agents that are effective at disinfecting smaller surfaces? Question 15 options: hydrogen perioxide steam Ultraviolet (UV) light Copper and/or silver coated surfaces All of the above Steam and UV light only
- If there is a sample having 3.5x10^5 CFU/ml, and this cell count came from a sample of the original culture which had been diluted 1x10^-4 times, what is the number of CFU/ml of the original culture?You begin with an overnight culture of approximately 108 organisms/ml and need a concentration of 103 bacteria/ml in order to get a countable spread plate. Describe how you would dilute your sample.Which piece of equipment sterilizes hospital tools and devices and how does it sterilize things? Are boxed gloves sterile?