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- Titration curve of an unknown amino acid The graph below shows a curve, which was obtained following titration of an unknown amino acid. Include a suitable descriptive title stating the identity of the unknown amino acid Use rectangles to precisely outline the regions in the graph where ionisable groups show buffering activity (base/acid ratio 1:10 to base/acid ratio 10:1); clearly associate the name of the ionisable group with the buffering regions; indicate within the graph all observable titration mid-points and all observable titration end-points and indicate estimates of the pKa of the three functional groups (do not provide pka values from the literature, you need to read the pka from the titration curve provided). Note that it is not possible to estimate the pka with more than one decimal precision due to limited resolution of the shown graph.Ionic interactions are weaker in water than they are in an anhydrous medium. Describe how water reduces the strength of these interactions.The-carotene molecule has λmax 450 nm and ɛ = 15.000 m2 / mol. Calculate the expected absorbance value for a solution in which 0.1 mg is dissolved in 10 mL of water. ( β-carotene, C40H56, 536 g/mol path length 1 cm)