You need to prepare 500 ml PBS buffer, a solution commonly used in biological research. The buffer helps to maintain the pH, and the osmolarity, as well as ion concentrations of PBS buffer, match those of the human body. PBS buffer consists of the following components: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4. How would you prepare the buffer?

Concepts of Biology
1st Edition
ISBN:9781938168116
Author:Samantha Fowler, Rebecca Roush, James Wise
Publisher:Samantha Fowler, Rebecca Roush, James Wise
Chapter2: Chemistry Of Life
Section: Chapter Questions
Problem 6RQ: Using a pH meter, you find the pH of an unknown solution to be 8.0. How would you describe this...
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You need to prepare 500 ml PBS buffer, a solution commonly used in biological research. The buffer helps to maintain the pH, and the osmolarity, as well as ion concentrations of PBS buffer, match those of the human body. PBS buffer consists of the following components: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4. How would you prepare the buffer?

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