For each of the examples on this page, calculate approximate organism size. Determine the field of view based on the objective used to capture the image.
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Field of view is defined as the area under the microscope which is visible at one time.
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- Which of the following statements BEST represents the great plate count anomaly? a The aggregation of the microbial cells causes a problem in the accurate population estimate using plate count. b The colonies growing on a petri plate is not always the organism under the microscope. c The population count using viable cell count is only 1% of the real population. d The number of microbes seen under the microscope is greater that the colonies growing on a petri plate.1. Write in a summary paragraph, to identify the test/stain in Microbiology. 2. Write in a summary paragraph, to indicating observations of a positive result and a negative result for each test/stain. 3. Write in a summary paragraph, to explain what each positive result and each negative result mean interpretation for each test/stain. 4. Write in a summary paragraph, to identify the organisms removed from your possible identifications at each stage of the flow chart, and which organisms remain as possible identifications at these steps.Identification of an unknown microorganism involve (select all that apply). Accumulate information that pertains to the organisms' morphological characteristics. Accumulate information from tests that pertains to the organisms' biochemical and physiological characteristics. Accumulate information that pertains to the organisms' growth preferences.
- As shown in this diagram, you perform a ten-fold serial dilution of a culture to determine the number of colony forming units (CFU) per mL it contains. You do a plate count with these growth results (no. of colonies for each dilution): 1:10 too many to count; 1:100 too many to count; 1:1,000 312; 1:10,000 38; 1:100,000 no growth. The number of CFU per mL in the original culture was: A. 38 B. 380,000 C. 38,000 D. None of the other four answers (Correct answer not given) E. 10,000site your reference:) What are the basic concepts and precautions to be observed in specimen collection for Microbiological examination? Enumerate specific examples of clinical specimens collected for microbiological examination. Give 2 specific examples of bacteria isolated from clinical samples. What is a transport medium; give specific examples and state their purpose.Gloves are required for ALL standard laboratory procedures using BSL-1 organisms. TRUE or FALSE? provide reasoning Mouth pipetting is allowed in the BSL-1 lab when pipetting devices are not available. TRUE or FALSE? provide reasoning
- In a viable plate count, each _________ represents a _________fromthe sample population.a. cell, colony b. colony, cell c. hour, generation d. cell, generation3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV. A. The cell is being viewed under high power. (the pink specimen picture belongs to this question) Scanning power objective=4x; Low power objective= 10x; High power objective= 40x; Eyepiece= 5x; Low power field of view (FOV)= 4.2 mm B. These cells are being viewed under high power. Use the length of just one cell to estimate the number of cells that can fit into the FOV. (the two yellow circle specimen picture belongs to this question) Scanning power objective = 5X; Low power objective = 40X; High power objective = 100X; Eyepiece = 10X; Low power field of view (FOV) = 1.5 mmWhat are the basic differences between the three plating methods? Fill up the following table. Streak Pour Spread Equipment/ Materials used in immobilizing and separating cells Purpose(s) (isolation, enumeration, both) Type of colonies (surface, subsurface, both) Advantage Disadvantage
- Explain any 2 advantages the fully automated analyser has over the spectrophotometer.Which of the following statements is FALSE? Question options: increasing wavelength improves ones ability to view microscopic structures increasing resolution can improve ones ability to view microscopic structures increasing magnification can improve ones ability to view microscopic structures all of these statements re TRUE increasing contrast can imporove ones ability to view microscopic structures oil immersion can increase the resolution of light microscopes[2] Instructions: Read each excerpt from a research paper and identify the dependent, independent, controlled variable (at least 1), experimental group/treatment, and control group. Independent variable: Dependent variable: Controlled variable: Experimental Group: Control group: