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For the CRISPR/CAS system to function, DNA ligase must be added to the target cell.
a. true
b. false
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- Attached is a cartoon model of the CRISPR system, with 3 critical components labeled. A. What is the identity of each component? B. What is the function of component a? C. What is the function of component c?Write five applications of CRISPR/Cas system in diagnosis and therapy with examplesThe CRISPR-Cas9 system can be used to: a. sequence a genome in living cells b. edit a gene in living cells and organisms c. diagnose sickle-cell anemia d. identify a specific gene e. detect gene expression under different conditions
- Which of the following can be a achieved by CRISPR/Cas9-based genome editing tool? A. Knocking out one or more gene B. Activating expression of a gene C. Suppressing expression of a gene D. All of the aboveBriefly describe how the CRISPR/Cas9 system modifies genomic DNA and why this can help treat genetic disease. Make it understandableDirect modification of genes may be achieved using what? (Choose one below): DNA microarray CRISPR-Cas system Hybridization
- The BLAST program begins with a particular genetic sequence anda. translates it into an amino acid sequence.b. determines if it contains one or more genes.c. identifies homologs within a database.d. does all of the above.write the pros and cons of crispr technology in bullet points with explaintionWhich of the following is a concern of in-vivo gene therapy? A. Lytic vector expression B. Integration into target region C. Immune response to viral vector D. Production of chimera
- Why is genome editing by CRISPR-Cas advantageous over traditionalmethods for creating knockout or transgenic animals?Explain your answers.Which of the following methods is used to detect a specific RNAwithin a mixture of many different RNAs?a. Site-directed mutagenesis c. Western blottingb. Northern blotting d. None of the aboveYou have extracted a long piece of DNA from a human cell and you want to extract the gene of interest for you to clone it. Assuming that you know the sequence of the DNA what methods can youuse to amplify the gene of interest? A. antibody purification B. polymerase chain reaction C. none of the above D. ligation