Q: CRISPR is a tool used by bacteria to fight virus attacks. In your own words, describe how CRISPR…
A: CRISPR, used as a defense tool by bacteria to fight virus attacks.
Q: Draw the PCR machine with marking in detail
A: Polymerase chain reaction is method which is widely used to make billions of copies of the DNA…
Q: In recombinant DNA technology, restriction enzymes cleave the DNA at a specific site known as:
A: Restriction enzyme, also known as restriction endonuclease, is a bacterial protein that cleaves DNA…
Q: What is southern blot? Understand PCR reaction in detail. What polymerase enzyme is used in PCR…
A: Both southern-blotting and PCR are types of recombinant DNA technology. Recombinant DNA technology…
Q: DNA can be separated based on fragment size using ________.
A: DNA is Deoxy-Ribo-Nucleic acid and is made by two polynucleotide chains coiled together Structure…
Q: DNA extraction from banana is being analyzed
A: The stringy substance that you see is DNA! It has been removed from the millions and millions of…
Q: Draw an Illustration of the steps in restriction digestion and PCR [ Please make it clean and…
A: Restriction digestion is the process of cutting the DNA sequence at a particular nucleotide sequence…
Q: make a diagram or schematic representation of Restriction fragment length polymorphism or RFLP
A: Genetics is a part of science worried about the investigation of genes, genetic variety, and…
Q: Identify a problem in molecular biology and how to use CRISPR/cas 9 system to solve it.
A: "CRISPR" is "clustered regularly interspaced short palindromic repeat". It's a piece of DNA that…
Q: Write five applications of CRISPR/Cas system in diagnosis and therapy with examples
A: CRISPR-Cas9 is genome editing tool that enables geneticists medical researchers to edit parts of the…
Q: Describe the nature of recognition sites for restriction enzymes and the nature of the ends of the…
A: Vast uses of endonuclease activity of restriction enzyme found in the biotechnology for gene…
Q: Aside from Sanger Sequencing, what are the other DNA sequencing methods that are available today?…
A: Sanger sequencing is a DNA sequencing technology that uses electrophoresis and is based on DNA…
Q: What is the fastest method to determine if a genetic disorder is due to a mutation at a palindromic…
A: Mutations This refers to the alterations in the sequence of DNA. These are caused from the mistakes…
Q: What is the benefit of using a strain of Lactobacillus bacterium that contains the CRISPR locus in…
A: CRISPR is a group of DNA sequences present in the genomes of archaea and bacteria, which are…
Q: Describe the basic components of CRISPR.
A: CRISPR mechanism is useful for the bacteria in protecting themselves from the attack of viruses. It…
Q: The detection of single nucleotide polymorphisms is possible with: O A. Restriction Enzymes O B. All…
A: SNPs or single nucleotide polymorphisms are the most common type of genetic variation in humans.…
Q: Create your own concept map on vectors used in rDNA technology
A: The combining of DNA molecules form two different species is known as recombinant DNA technology.…
Q: Sanger sequencing Write a precise and accurate differential report on the above sequencing…
A: DNA sequencing is the process of determining the sequence of nucleotides (As, Ts, Cs, and Gs) in a…
Q: What are three benefits Ion torrent sequencing has over sanger sequencing
A: DNA is an important biomolecule in the cell as it is the genetic material for most of the organisms…
Q: What type of probe is used for real-time PCR? Explain howthe level of fluorescence correlates with…
A: In the conventional amplification technique of PCR (polymerase chain reaction), the amplified…
Q: Construct a detail linear restriction map according to the given information in Table 1 below. Table…
A: Total size of DNA= 11 kb EcoRI has one restriction site cutting it into 2 fragments of 2kb and 9kb…
Q: Suggest and describe two methods to detect DNA concentration by using in text citation and provide…
A: DNA is genetic material in almost all living organism that transit from one generation to another
Q: Explain how CRISPR was discovered and developed into the tool available today.
A: CRISPR stands for clustered regularly interspaced short palindromic repeats. These were naturally…
Q: Outline the steps by which the polymerase chainreaction (PCR) amplifies a specific region of a…
A: Polymerase chain reaction or PCR technique is used to amplify or make multiple copies of a specific…
Q: Explain how oligonucleotide primers in the Polymerase Chain Reaction work (PCR)
A: PCR or polymerase chain reaction is an in-vitro DNA amplification technique. It is used to amplify…
Q: Restriction Enzymes with LongerRecognition Sites Produce Larger _______ Fragments
A: The restriction enzyme is the molecular scissors that cut at a specific site. These are naturally…
Q: Identify the mRNA sequence that encodes the protein Design primers that will allow them to amplify…
A: Deoxyribonucleic acid(DNA) is a molecule comprised of two polynucleotide chains coiled around each…
Q: SANGER SEQUENCING NEXT GENERATION SEQUENCING WRITE A PRECISE AND ACCURATE DIFFERENTIAL REPORT ON…
A: sanger sequencing and next-gen sequencing is that the sanger method only sequences a single DNA…
Q: Short tandem repeats (STR) profiling is based on
A: Small tandem repeats (STRs) are short repeating DNA sequences (2–6 bp) that make up roughly 3% of…
Q: what do you think about the ethical impacts of a technology such as crispr
A: Step 1 CRISPR (Clustered regularly interspaced short palindromic repeats) works like a pair of…
Q: CRISPR-cas9 is a powerful technique for gene editing. Please place the stages of a CRISPR-cas9 gene…
A: Design a set of targetting sequences using algorithms. For successful genome editing, the major…
Q: Describe the purpose and process of DNA finger printing.
A: DNA fingerprinting is the method of recognizing the genetic makeup of individuals through the…
Q: Explain two parameters that should be considered when designing primers for PCR.
A: PCR stands for Polymerase Chain Reaction. Using this technique, a fragment of DNA can be amplified.…
Q: When using blunt-end primers, how would you determine the correct plasmid construct from the…
A: Ligation is the joining or ligation of two nucleic acid fragments through the action of a ligase…
Q: Briefly describe what CRISPR technology does
A: Gene is a particular segment of deoxyribose nucleic acid (DNA) that is responsible for the…
Q: Illustrate the steps in restriction digestion and PCR
A: Polymerase chain reactions is a technique used to make millions of copies rapidly of a DNA sample.…
Q: A DNA fingerprint consists of sections of DNA that have been cut with restriction endonuclease…
A: DNA fingerprinting DNA fingerprinting is a modern technique that help us to solve crimes. The…
Q: List the steps of a PCR cycle
A: DNA contains inheritable segments that are called genes that contain instructions for protein…
Q: The CRISPR system may be used to manipulate the genome in human cells, because Cas9 can
A: CRISPER system can use Cas protein to manipulate the genome present in human cell.
Q: Discuss the principle ,uses, advantages and disadvantages of shotgun sequencing.
A: DNA sequencing are very essential to delineate the exact order and arrangement of nucleotides within…
Q: Explain the difference between a DNA profile and a microrarray.
A: Deoxyribonucleic acid (DNA) analysis is an important biotechnological tool that has vast…
Q: Briefly explain the rationales of adding chemicals which can affect DNA stability in polymerase…
A: PCR is widely used to rapidly make millions to billions if copies of a specific DNA sample.
Q: describe the two main parts of a plasmid DNA extraction and purification procedure including the…
A: Plasmid DNA extraction
Q: Explain why the 16S rRNA gene sequencing is suitable for bacterial identification in general and why…
A: The Prokaryotes have a 70S type of ribosome (S=Svedberg units). The 70S ribosome is made up of a 50S…
Q: Cite three examples of an application of PCR (polymerase chain reaction).
A: Three examples of an application of PCR (polymerase chain reaction) - 1. Amplification of the DNA /…
Q: using the process flow of PCR describe the principle associated with the PCR technique.
A: Introduction : Polymerase Chain Reaction or PCR is a widely employed technique in molecular biology…
Q: Draw a diagram illustrating a bacterial CRISPR locus. Label your drawing with a brief description of…
A: CRISPR is a family of DNA sequences that can be found in the genomic section of the prokaryotic…
Q: Describe how Ion torren method of sequencing works in detail.
A: Thermo Fisher Scientific's Ion Torrent technology brings a whole new method to Next Generation…
Q: Describe the three steps of a PCR cycle.
A: Polymerase chain reaction is a molecular biology technique used to amplify a single copy of DNA that…
Q: Explain what gene editing using CRISPR means.
A: Given: CRISPR gene editing.
write the pros and cons of crispr technology in bullet points with explaintion
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- True or False : CRISPR is a novel method for reliably amplifying gene products so that they can be sequenced and studied.Briefly and in simple terms, describe how CRISPR could be utilized in treating genetic conditions such as Batten disease.Explain briefly within 5 sentences each 1. a. What is PCR? Explain/relate its importance in DNA marker analysis. 1.b. What are restriction enzymes (RE)? Describe how a RE can be used to develop/design a DNAmarker.