For what purpose would you adjust each of the following microscopecomponents during a microscopy exercise?a. Iris diaphragm:b. Coarse-adjustment knob:c. Fine-adjustment knob:d. Condenser:e. Mechanical stage control:
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For what purpose would you adjust each of the following microscope
components during a microscopy exercise?
a. Iris diaphragm:
b. Coarse-adjustment knob:
c. Fine-adjustment knob:
d. Condenser:
e. Mechanical stage control:
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- a. Do you open both eyes or close one eye when viewing a specimen under a microscope? Why? Why not? b. In using a compound microscope, do you start with the low power objective (LPO) or the high power objective (HPO)? Why or Why not? c. When using 60X, do you it together with the coarse adjustment knob or fine adjustment knob? d. Why must you slowly turn the fine adjustment knob? e. Is natural light or is artificial light used when using the microscope?Define each of the following terms: A) What is resolution and how is resolution related to the wavelength of light used to illuminate the sample? B) What is the magnification of the specimen if you are using a 40x objective and a 10x eyepiece? C) How is the numerical aperture (NA) of a lens related to its ability to gather light from a specimen?What are the advantages of using a Refractometer instead of other Specific Gravity measurement equipment used in Clinical Microscopy?
- In light microscopy, when magnification is increased, the lens focuses closer to the sample. This makes the user more likely to ram the sample into the lens. Why is it common for the lens to focus closer to the sample at higher magnifications? What can be done to mitigate this risk?Which of the following will decrease the limit of resolution (Select all that applies) a) using lower wavelength of illumination b) using an objective lens with lower numerical aperature c) using immersion oil d) using electron as the source of illumination instead of lighta. Why must you slowly turn the fine adjustment knob? b. Is natural light or is artificial light used when using the microscope?
- Contrast how the views differ under high power (40x) and oil immerson (100x) objective lenses in terms of a) magnification b) detail of the cell, c) size of the area being viewed and d) resolution.Why did we calculate, rather than measure, the diameter of the field of view on medium and high power? When using the compound microscope, you always begin viewing your specimen on __________ power, and use the __________ focus knob.If accessories for darkfield microscopy were not available, how would you construct a simple star diaphragm?
- Differentiate between the limit of resolution of the typical light microscope and that of the unaided human eyes the relationship between the depth of field and magnification is that When magnification increases, the depth of field decreases. Name two procedures (steps) you should do to achieve the maximum resolution when using the oil immersion lens State the relationship between the working distance of an objective lens and its magnification power “The coarse focus knob can be used to adjust the focus when using any of the objective lenses”. Is this statement true or false? If it is false, please correct it. When using the oil immersion lens but you cannot focus, what should you should not do? And what you should do? What does this statement mean: “Your microscope is parfocal”? What is the significance of condenser lens? Multiple choice question: The most useful adjustment for increasing image contrast in low power magnification is _______ Closing down the diaphragm Closing one…The girl is focusing a slide and she is turning the coarse adjustment knob up toward the slide. A. What has happened to the slide? B. Is this the proper way to focus? C. Should you focus by turning the coarse adjustment up or down? D. What happens to a microscope if place at the edge? E. What should you do to present this from happening?How can you improve contrast when viewing a specimen?. What should you do if you have difficulty focusing in high power?. Why is important to use the fine adjustment nob only when focusing while using the high dry objective lens?