Which of the following is a parameter of microscopy that can affect your ability to resolve a specimen using a compound light microscope? Question options: Contrast Resolving power All of the choices Numerical aperture
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Which of the following is a parameter of microscopy that can affect your ability to resolve a specimen using a compound light microscope?
Question options:
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Contrast
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Resolving power
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All of the choices |
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Numerical aperture |
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- Which of these is NOT a best practice when using, handling, or storing a microscope and microscope slides? Do not touch the objective lenses. Store the slides in bright sunlight to prevent mold growth. Rotate the nosepiece to the lowest objective before storing the microscope. Keep dust away from the scope by covering it during storage.Which of the following statements are true in the context of the microscope's field of view? I. It is the observable area within a circular space as defined by the lenses II. FOV is the smallest on the lowest power objective III. FOV is the maximum area visible through the microscope eyepiece IV. When you switch to a higher power, the field of zooms out towards the centerWhich of the following is true about the relationship between depth of field and magnification? There is no relationship. When magnification increases, depth of field decreases. When magnification increases, depth of field increases. Stereomicroscopes offer a lesser depth of field than compound microscopes. The depth of field of a microscope determines how much magnification it can achieve.
- A student using a microscope measured the low power field diameter to be 7.1mm. The student then switched from the low power (4x) objective lens to the medium-power (10x) objective lens. Assuming a 10 x ocular (eyepiece) lens, the medium power field diameter is ____ um um is micrometersThe compound (bright-field) microscope is parfocal. It means that: Question 4 options: objective lenses are built into revolving nosepiece its lenses effectively decrease focal lenght can achieve maximum resolution lower than 0.2 μm image should remain in focus when objective lens is changedHow will the following affect resolution during microscopy? I) Closing or opening the diaphragm II) Raising or lowering the condenser III) Increasing or reducing the light intensity
- The objective lenses of the microscopes used in this course are all parfocal. What does having parfocal objective lenses mean? Question options: The slide has to be moved away from the objective lens (i.e., lowered) when switching between objective lenses. None of these choices The objective lenses have optimized light gathering capability. Once in focus, you may switch between objective lenses with only fine focus adjustment required.What part of the microscope is used to make small-scale adjustments to the height of the stage? Group of answer choices 1. fine focus 2. iris diaphragm 3. eye pieces 4. coarse focus 5. slide holder adjustmentWhen trying to focus an object on the microscope, you always begin with the ________________ objective lens and use __________________ adjustment knob to bring the object in focus. Once object is focused, high power objective lens can be used to magnify the object, and only use the ___________________ adjustment knob to focus at this point. a scanning; coarse; fine b low power; coarse; fine c low power; fine; coarse d scanning, fine; coarse
- In light microscopy, when magnification is increased, the lens focuses closer to the sample. This makes the user more likely to ram the sample into the lens. Why is it common for the lens to focus closer to the sample at higher magnifications? What can be done to mitigate this risk?What is the highest magnification of the following microscopes?Bright fieldDark fieldPhase-contrastFluorescenceConfocalScanning EMTransmission EMScanned-ProbeAs with standard phase contrast microscopy,differential-interference contrast microscopy depends on differences in the ___________________ of a speciment to create high-contrast images. a. refractive index b. light absorption c. natural fluorescence d. walls and coverings e. natural pigmentation
