Give answer to all parts? 1. Why is the elimination of DNA or protein is important in RNA isolation? 2. Why is the integrity of isolated RNA an important aspect of RNA isolation?
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Give answer to all parts?
1. Why is the elimination of DNA or protein is important in RNA isolation?
2. Why is the integrity of isolated RNA an important aspect of RNA isolation?
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Solved in 5 steps
- Note: Answer the number 3 question only. Explain. 1. What is the Difference between DNA Purification and RNA Purification? 2. why contaminants must be avoided in DNA samples? 3. Give 5 examples of sources of error in using a spectrophotometer.Note:Answer the number 2 question only... 1. What is the Difference between DNA Purification and RNA Purification? 2. why contaminants must be avoided in DNA samples? 3. Give 5 examples of sources of error in using a spectrophotometer.n gel electrophoresis of DNA, the different bands in the final gel form because the DNA molecules _______. a. are from different organisms b. have different lengths c. have different nucleotide compositions d. have different genes
- Explain. 1. What is the Difference between DNA Purification and RNA Purification? 2. why contaminants must be avoided in DNA samples? 3. Give 5 examples of sources of error in using a spectrophotometer.Define nucleic acid. What are the best sample sources that can be used in the extraction of nucleic acids? Give examples (2) of nucleic acids. Differentiate RNA from DNA in terms of their chemical properties.MULTIPLE CHOICE Read the questions below carefully. There is only one correct answer for each question. (no explanation only correct letter needed) 1. In a DNA sample of Escherichia coli (a bacteria), 23.6% of the nitrogenous bases are thymine. What percentage of cytosine nitrogen bases is present in this sample? a) 76.4% b) 23.6% c)26.4% d) 52.8% 2. If the adenine is located on the Z strand as shown (see attachement 3) , then on the W strand, in the same place, we should find: a) Uracil b) Adenine c) Thymine d)Cytosine 3. Which of these deletions best represents a chromosomal deletion? (see options in attachment 3) 4. It is known that RNA is a nucleic acid responsible for the synthesis of proteins. However, there is another nucleic acid alongside RNA: DNA. What role does the latter play in relation to RNA? a) RNA is synthesized in the cell in case too large a mutation damages the DNA. b) DNA has the reproductive genetic code of all cells and passes it on to RNA. c) DNA directs the…
- MULTIPLE CHOICE Read the questions below carefully. There is only one correct answer for each question. 1. In a DNA sample of Escherichia coli (a bacteria), 23.6% of the nitrogenous bases are thymine. What percentage of cytosine nitrogen bases is present in this sample? a) 76.4% b) 23.6% c)26.4% d) 52.8% 2. If the adenine is located on the Z strand as shown (see attachement 3) , then on the W strand, in the same place, we should find: a) Uracil b) Adenine c) Thymine d)Cytosine 3. Which of these deletions best represents a chromosomal deletion? (see options in attachment 3) 4. It is known that RNA is a nucleic acid responsible for the synthesis of proteins. However, there is another nucleic acid alongside RNA: DNA. What role does the latter play in relation to RNA? a) RNA is synthesized in the cell in case too large a mutation damages the DNA. b) DNA has the reproductive genetic code of all cells and passes it on to RNA. c) DNA directs the synthesis of enzymes, while RNA synthesizes…Give only typing answer with explanation and conclusion A DNA ligase: None of the listed answers Can join two fragments of DNA binding the phosphodiester backbone with covalent bonds. All of the listed answers -not the correct answer Can recognize specific sequences that allow for the detection of SNPs. Can replicate a complementary DNA strand using a single stranded DNA template.Give typing answer with explanation and conclusion 5. The following diagram represents DNA that is part of the RNA coding sequence of a transcription unit. The bottom strand is the template strand. Give the sequence found on the RNA molecule transcribed from this DNA & identify the 5ʹ and 3ʹ ends of the RNA. 5′–ATAGGCGATGCCA–3′ 3′–TATCCGCTACGGT–5′ ← Template strand
- Bacterial DNA has how many origins of replication? A) 0 B) 1 C) 10 D) depends on the size of the DNAThe diagram shows an autoradiograph of a DNA sequencing gel. What is the 5' to 3' sequence of the template strand ("inferred strand") based on the pattern in this gel?What is the role of CTAB in DNA isolation? A. Neutralize negative charge of DNA B Precipitate DNA C Remove polyphenols and tannins D Precipitate polysaccharides