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Give atleast 3 differences between UV and PDA detector and explain
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- What does the abbreviaton "EI" stand for? Group of answer choices Aearly ionization Belectron imaging Celectromagnetic induction Delectron intensity Eelectron ionization Which of the following transitions will exhibit the shortest wavelength in the UV-Vis spectrum? Group of answer choices An to p* Bs to s* Cp to s* Dn to s* Ep to p*How is the UV spectrometer work? What is absorbance and how is it determined?What method do ELISA’s utilize in their measurement? a) light absorption (eg Abs480nm) b) b+–radiation c) combination of absorption and radiation d) electrophoresis
- What are the light sources used in UV-vis spectrophotometry.give ansDefine stimulated emission depletion (STED) microscopy technique.Suppose you measure the scanning (4x) field of vision diameter with a stage micrometer and it is 2 mm. If high power is 40X, and gives 10X more magnification than scanning power, how big will the diameter of the field of vision be using high power?
- Use the iris diaphragm to attain the best illumination of the letter “e” on the scanning (4x) objective. Open and close the iris diaphragm and notice the changes in contrast of the letter “e”. How did the appearance of the letter “e” change under the various levels of illumination? 1 Repeat the above procedure on the high-power (40x) objective. When increasing magnification, what happens to the light available? Why?If we used the 365nm setting on the UV light box would this have caused more or less UV light damage, explain why?Give a specific example on how to use UV-Vis spectrophotometer in medtech-medical field. Provide a procedure on how to do this example that you provided. Explain the procedure and what data would you gather?