5A biochemist wants to separate Glu, Arg, and His using a cation-exchange chromatograpy. Determine the order of elution from this column using pH=7.00 buffer mobile phase. A biochemist wants to separate Glu, Arg, and His using a cation-exchange chromatograpy. Determine the order of elution from this column using pH=7.00 buffer mobile phase.O Glu is first, His is second, and Arg is last to elute from the column.His is first, Arg is second, and Glu is last to elute from the column.Arg is first, His is second, and Glu is last to elute from the column.O Glu is first, Arg and His will elute last at the same time from the column.Arg and His will elute is first at the same time, Glu is last to elute from the column.

In this question we have to choose Correct option of the separate Glu, Arg and His using a cation -…

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Glu is first, His is second, and Arg is last to elute from the column.

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter33: High-performance Liquid Chromatography

Section: Chapter Questions

Problem 33.2QAP

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Question

5 A biochemist wants to separate Glu, Arg, and His using a cation-exchange chromatograpy. Determine the order of elution from this column using pH=7.00 buffer mobile phase.

A biochemist wants to separate Glu, Arg, and His using a cation-exchange chromatograpy. Determine the order of elution from this column using pH=7.00 buffer mobile phase.
O Glu is first, His is second, and Arg is last to elute from the column.
His is first, Arg is second, and Glu is last to elute from the column.
Arg is first, His is second, and Glu is last to elute from the column.
O Glu is first, Arg and His will elute last at the same time from the column.
Arg and His will elute is first at the same time, Glu is last to elute from the column.

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