Gradient centrifugation is commonly used for which of the following? separating organelles out of a homogenate measuring the concentration of proteins in a solution separating proteins in a solution isolating small molecules

Gradient centrifugation is commonly used for which of the following? separating organelles out of a homogenate measuring the concentration of proteins in a solution separating proteins in a solution isolating small molecules

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter12: Gravimetric Methods Of Analysis

Section: Chapter Questions

Problem 12.2QAP

See similar textbooks

Related questions

Q: Which of the following is incorrect? a. (-) charged proteins bind to (+) charged solid supports b.…

Q: Define the following terms :(i) Kraft temperature(ii) Peptization(iii) Electrokinetic potential

A: Surface chemistry is a phenomenon occurring between two different surfaces.

Q: In what buffer system (PAH, SAD, or DOH) will Phoebe store the enzyme? (show calculations and…

A: The pH of a buffer solution can be determined by Henderson's equation given below. pH=pKa +…

Q: 8.30 The diffusion coefficients for a lipid in a plasma membrane and in a lipid bilayer are 1.0x…

A: Solution - Accoeding to the question - Given - t for plasma membrane= 1 x 10-6 sec. t for lipid…

Q: Which of the following parameter correlate inversely with surfactant penetration into skin? Micelle…

A: As per the rules only the first question can be answered:

Q: Coprecipitation that involves foreign particles? a. Inclusion b. Mechanical entrapment c. Surface…

A: Co-precipitation :– The concurrent precipitation of more than one compound from an solution.…

Q: Name 5 different purification methods for cobalt(II) sulphate (CoSO4.7H2O)

A: The chemicals used for various purposes should be completely free from any type of impurities.…

Q: Each compound of the solute mixture is adsorbed with different forces, which causes it to move over…

A: In chromatography, the distribution of the components of the unknown mixture into two liquid phases…

Q: What other diseases/conditions can you find an increased LAP activity? Give a brief description of…

A: Following Diseases have an increased LAP activity these include 1.Nonleukemic neutrophilia - Here…

Q: Write with the reasons why a separation will occur when a mixture of polypeptides with isoelectric…

A: Isoelectric point (pI) is the pH value at which the positive charge equals the negative charge,…

Q: Please explain to me what is the valency factor (n-factor) of Potassium Bipthalate (C8H5KO4)

A: Potassium Bipthalate - It is also known as potassium hydrogen phthalate. It is the monosodium salt…

Q: A compound microscope has an ocular micrometer calibrated to 12μm for each observable division in…

A: A high-resolution microscope that employs two sets of lenses to provide a two-dimensional image of…

Q: QI. Shown below is the general structure of alanine. H3C OH NH2 a Without help from unauthorized…

Q: The Ksp of BaSO4 is 1.7 x 10-10. Calculate the concentration of the Ba+2 and SO4-2.

A: Since you have posted multiple questions, we will solve first one for you. To get remaining…

Q: Indicate the effect (increases, decreases, or stays the same) that each of the following changes…

A: A question based on analytical separations that is to be accomplished.

Q: The Ksp for BaF2 is 1.6x10^-6.What is tge concentration of Ba+2 ions in this solution?

A: The balanced equation for the dissociation of BaF2 is, BaF2 s ↔ Ba2+aq + 2F- aq…

Q: over- The Ez component of the PDC contains active sile(s) and. subunit/s- 2:2 different 2:2 idential…

A: The answer is attached below-

Q: Gas Chromatography (GC) is one of the useful techniques in analytical chemistry. Give two important…

A: It is one of the most convenient technique for the analysis and separation purposes of molecules in…

Q: 4. Why is the following relationship valid? heathte-heat.ater 5. What is the effect of increased…

A: We’ll answer the first question since the exact one wasn’t specified. Please submit a new question…

Q: What is the stationary phase in thin layer chromatography and its polarity? Choices: Cellulose -…

A: In thin layer chromatography(TLC) silica is used as stationary phase. Silica is a polar adsorbent .

Q: Describe Brownian motion in continuum transport. I want a clear solution for me to understand

Q: What is the key feature of a dialysis bag needed for it to be useful for measuring the binding…

A: Answer - Equilibrium dialysis is useful for measuring small molecule binding to macromolecule . It…

Q: 4) What is the K, for disodium asparaginate? asparagine: FM 132.119 TZ 2.00

A: Given: To determine: kb for disodium asparaginate.

Q: How do you calculate the retention factor (Rf) for each spot ? Distance traveled by spot O 5.5cm…

A: Calculating the retention factor as:

Q: Two different proteins X and Y are dissolved in aqueous solution at 37 ∘C. The proteins bind in a…

A: To calculate Kc , we would first write a reaction as mentioned above . Then we would draw an ICE…

Q: Vhich of these types of coprecipitation happens with greater probability when the ratio of the…

A: The type of coprecipitation happens with greater probability when the ratio of the contaminant…

Q: How long will it take for a S S protein to completely spin to the bottom of centrifuge tube filled…

A: b) is the correct answer 0.02 hours as other all three are in hours as the length of protien is in…

Q: 3. Isoelectric focusing is a separation technique that takes advantage of the differences in…

A: Isoelectric focusing: It separates the different amino acids based on their differences in their pI…

Q: 4. You are developing a GC method to separate xylene isomers (xylenes are benzene rings with two…

A: GC

Q: The best type of stationary phase for separation of negative ions is alumina C18/silica gel…

A: Negative ions are negatively charged ions. Negatively charged ions binds to only positively charged…

Q: Determine the concentration of Pb2+ in solution w/ 0.040 M NaNO3 that's satured with PbCl2 . (Ksp,…

A: The solubility product of PbCl2 = 1.66 × 10-5 Molarity of NaNO3 solution = 0.040 M

Q: (ii) CO₂Me 1) Zn/Cu, CH₂l2 2) Et₂0/reflux

Q: Calcylafe the contentrafion , epuilibrivm congtant

Q: The following data apply to a 1g sample of a granular powder Volume of the solid alone = 1 cm3/2g…

A: Given: Volume of the solid alone = volume of grain = 1 cm3/2g Volume of intraparticle pores = pore…

Q: what is not a direct method o determine the size of a bacterial population?

A: The measurement of the exact thing that is looking for the measurement is termed as direct…

Q: The osmotic pressure of a protein solution was measured at 25°C. The following data were obtained. c…

Q: 2. Describe the underlying principles behind the complexometric determination of water hardness.…

A: Water is pure when nothing is added to it, but it becomes hard when the calcium and magnesium ions…

Q: What is the effect of prolonged digestion in gravimetric analysis?

A: Gravimetric analysis is the process in which the amount of an analyte in a sample is determined by…

Q: Consider an imaginary bacterial cell that contains equal concentrations of 900 different enzymes in…

A: Given Data: Molar Mass of Protein or Enzyme = 120000g/mol Specific Gravity of Cytosol = 1.25 % by…

Q: Why do most people elute bound proteins from an ion- exchange column by raising the salt…

A: Why do most people elute bound proteins from an ion- exchange column by raising the salt…

Q: 3. Distinguish between electrode and membrane polarizations. Which one is associated with ore…

Q: List all possible methods for the quantitative determination of glucose solution: A) Polarimetry B)…

Q: The amino acid glycine (H2N—CH2—COOH) can participatein the following equilibria in…

A: EXPLANATION: A) part Glycine behaves in equilibrium with H2O. H2N—CH2—COOH + H2O ⇌ H2N—CH2—COO- +…

Q: You would like to calculate the concentration of a protein in a solution measured in a 96 well…

Concept explainers

Mass Spectrometry

This process is an instrumental method in which a sample is changed to fast moving positive ions by electron bombardment. The charged particles vary due to their different mass to charge ratio and are estimated with the help of this difference. It is also called mass spectroscopy.

Question

Gradient centrifugation is commonly used for which of the following?
separating organelles out of a homogenate
measuring the concentration of proteins in a solution
separating proteins in a solution
isolating small molecules

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 2 steps with 1 images

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

The concentration of a biomolecule inside a rod-shaped prokaryotic cell is 0.0043M. Calcuate the numer of molecules inside the cell which is 2.8 micrometers long and 1.4 micrometers in diameter.
The act of a white blood cell engulfing a bacterium is ______. phagocytosis diffusion exocytosis osmosis
The activity coefficient of aqueous glucose (C6H12O6) is ______. cannot be estimated from the given information slightly more than 1 slightly less than 1 much less than 1 0 exactly 1
Two-dimensional gel electrophoresis of proteins in a cell extract provides a qualitative way to compare proteins with respect to intracellular abundance. Describe a quantitative approach to determine the number of molecules of an enzyme per cell.
Define chemiluminescence.
The Ksp for BaF2 is 1.6x10^-6.What is tge concentration of Ba+2 ions in this solution?
How would you make 2.0 L of electrotransfer buffer for transferring the proteins from a gel onto nitrocellulose? Its composition is the following in water: 25 mM Tris (this is a solid; MW = 121) 192 mM Glycine (this is a solid; MW = 75.07) 20.0 % Methanol (you have 100% from the stockroom)
You are separating proteins on a cation exchange column. You need to be sure that the proteins are in a solution that is [> 2.0 pH units below, or 0.5 - 1.5 pH units above or 0.5 - 1.5 pH units below, or> 2.0 pH units above] the pI values of the proteins. You can elute the proteins from the column by using a mobile phase gradient where the %NaCl in the eluent gradually ["increases, decreases"] .
In the paper chromatography experiment, the filter paper is the stationary phase and water is the mobile phase False, cellulose is the mobile phase and salt solution is the stationary phase False, adsorbed water is the stationary phase and salt solution is the mobile phase False, salt solution is the mobile phase False, adsorbed water is the mobile phase and salt solution is the stationary phase
Define the following terms :(i) Kraft temperature(ii) Peptization(iii) Electrokinetic potential
What happens when a mixture of positively-charged Protein A and negatively-charged Protein B is loaded onto an anion-exchange column? Both proteins bind to the resin. Neither protein binds to the resin. Protein A binds to the resin and Protein B remains unbound. Protein B binds to the resin and Protein A remains unbound
what is not a direct method o determine the size of a bacterial population?